Candidate's Abstract) The long term scientific goal of this proposal is to identify and functionally characterize classes of genes that are involved in the mammalian response to 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure. A gene trap screen in mouse embryonic stem (ES) cells is proposed to do this in a efficient way. This experimental strategy simultaneously identifies novel genes, mutates them, tags them with a reporter molecule, and enables the production of knockout mice carrying the induced mutations. Within the proposed research period, it will be possible to conduct preliminary molecular and functional characterization of the role of these genes in cell cultures and animals. The knockout animals generated by this work will be useful for a variety of future investigations into the effects of environmental agents on mice and humans.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Career Transition Award (K22)
Project #
5K22ES000345-03
Application #
6489787
Study Section
Special Emphasis Panel (ZES1-LKB-C (01))
Program Officer
Shreffler, Carol K
Project Start
2000-01-15
Project End
2003-12-31
Budget Start
2002-01-01
Budget End
2003-12-31
Support Year
3
Fiscal Year
2002
Total Cost
$108,000
Indirect Cost
Name
Colorado State University-Fort Collins
Department
Public Health & Prev Medicine
Type
Schools of Veterinary Medicine
DUNS #
112617480
City
Fort Collins
State
CO
Country
United States
Zip Code
80523