application) The purpose of this application is to secure funding for partial salary support for the named P.I. to relieve him from some of the clinical responsibilities that currently provide salary. This would allow more time to perform patient oriented research outlined in this proposal and mentoring post-doctoral fellows at the Center for Human Nutrition (CHN) at the University of Colorado Health Sciences Center (UCHSC). The P.I. is well positioned to serve these roles in that he has a history of clinical research in the area of human obesity, has recently received independent funding for human nutritional studies, and is currently serving as the Associate Director for Research Training at the CHN under the direction of Dr. James Hill. The presence of the General Clinical Research Center (GCRC), the Clinical Nutrition Research Unit (CNRU), and the CHN have made the UCHSC an exciting institution for obesity related research and has attracted a large number of excellent post-doctoral fellows but there is a need/opportunity for a mid [sic] career MD to help with the mentoring load. This award would solidify the candidate in this role at an important time for him and the CHN. The proposed research examines the hypothesis that as compared to individuals predisposed to weight gain, lean individuals more accurately sense and respond metabolically and behaviorally to positive energy balance. It is hypothesized that as compared to reduced obese, lean individuals will respond to a period of positive energy balance with a reduction [sic] the oxidation of glucose (insulin resistance), an increase in the oxidation of dietary fat by skeletal muscle, and a decreased drive to eat. The result of these differences in fuel metabolism are that lean individuals will more effectively defend against a state of positive fat balance as compared to reduced obese. To test these ideas lean and reduced obese individuals will be studied following 3-days of overfeeding. 1. Euglycemic clamps will be performed to examine insulin sensitivity, 2. meal studies will be performed examining the metabolic fate of labeled fat and carbohydrate included in a test meal, and 3. Hunger, satiety, and palatability will be measured during and following hypercaloric feeding. Indirect calorimetry, tracer oxidation, limb balance, and sampling of skeletal muscle and adipose tissue are proposed to give a more complete view of meal associated nutrient metabolism in these two groups. These studies will provide insight into the biology that predisposes to weight regain following weight reduction and will lay the groundwork for future studies of the abnormalities in nutrient metabolism that exist in obese and pre-obese individuals.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Midcareer Investigator Award in Patient-Oriented Research (K24)
Project #
5K24DK002935-04
Application #
6692633
Study Section
Diabetes, Endocrinology and Metabolic Diseases B Subcommittee (DDK)
Program Officer
Podskalny, Judith M,
Project Start
2001-05-01
Project End
2005-12-31
Budget Start
2004-01-01
Budget End
2004-12-31
Support Year
4
Fiscal Year
2004
Total Cost
$123,480
Indirect Cost
Name
Denver Health and Hospital Authority
Department
Type
DUNS #
093564180
City
Denver
State
CO
Country
United States
Zip Code
80204
Creasy, Seth A; Rynders, Corey A; Bergouignan, Audrey et al. (2018) Free-Living Responses in Energy Balance to Short-Term Overfeeding in Adults Differing in Propensity for Obesity. Obesity (Silver Spring) 26:696-702
Rynders, Corey A; Bergouignan, Audrey; Kealey, Elizabeth et al. (2017) Ability to adjust nocturnal fat oxidation in response to overfeeding predicts 5-year weight gain in adults. Obesity (Silver Spring) 25:873-880
Pereira, Rocio I; Low Wang, Cecilia C; Wolfe, Pamela et al. (2016) Associations of Adiponectin with Adiposity, Insulin Sensitivity, and Diet in Young, Healthy, Mexican Americans and Non-Latino White Adults. Int J Environ Res Public Health 13:ijerph13010054
Hernandez, Teri L; Bessesen, Daniel H; Cox-York, Kimberly A et al. (2015) Femoral lipectomy increases postprandial lipemia in women. Am J Physiol Endocrinol Metab 309:E63-71
Bessesen, Daniel H; Cox-York, Kimberly A; Hernandez, Teri Lynn et al. (2015) Postprandial triglycerides and adipose tissue storage of dietary fatty acids: impact of menopause and estradiol. Obesity (Silver Spring) 23:145-53
Schmidt, Stacy L; Bessesen, Daniel H; Stotz, Sarah et al. (2014) Adrenergic control of lipolysis in women compared with men. J Appl Physiol (1985) 117:1008-19
Bergouignan, Audrey; Kealey, Elizabeth H; Schmidt, Stacy L et al. (2014) Twenty-four hour total and dietary fat oxidation in lean, obese and reduced-obese adults with and without a bout of exercise. PLoS One 9:e94181
Schmidt, S L; Kealey, E H; Horton, T J et al. (2013) The effects of short-term overfeeding on energy expenditure and nutrient oxidation in obesity-prone and obesity-resistant individuals. Int J Obes (Lond) 37:1192-7
Schmidt, Stacy L; Harmon, Kristin A; Sharp, Teresa A et al. (2012) The effects of overfeeding on spontaneous physical activity in obesity prone and obesity resistant humans. Obesity (Silver Spring) 20:2186-93
Harmon, Kristin A; Gerard, Lori; Jensen, Dalan R et al. (2011) Continuous glucose profiles in obese and normal-weight pregnant women on a controlled diet: metabolic determinants of fetal growth. Diabetes Care 34:2198-204

Showing the most recent 10 out of 22 publications