The major goal of this Mid-Career Investigator Award in HIV related Patient-Oriented Research is to use the resulting protected time and effort to mentor a new generation of junior investigators at Brown University and its affiliated hospitals in translational studies of HIV pathogenesis and transmission. Mentees will be recruited from a robust educational infrastructure that includes several NIH funded HIV-centric programs including training grants (T32DA013911, T32MH078788, T32AA007459), international AIDS training and research programs (D43TW000237, D43TW009597) and Center grants including the Lifespan/Tufts/Brown CFAR (P30AI042853) and the Brown University Alcohol Research Center on HIV (P01AA019072). A recently funded 5-year research grant (R01HD072693) will be used as an immediately available foundation for mentoring and will allow instruction in patient oriented research that connects the clinic and laboratory with the pursuit of the following specifi aims (Aim 1) To quantify the effect of exogenous and endogenous reproductive hormones on HIV susceptibility of the reproductive tract of pre- and post-menopausal women.
(Aim 2) To determine if reproductive hormones alter mechanisms responsible for HIV transmission through cervicovaginal epithelia.
(Aim 3) To quantify the effect of reproductive hormones on HIV dependency factors in the female genital tract. The K24 mechanism will be used to support a new aim involving the longitudinal study of the cervicovaginal tract of adult women victims of sexual violence with the goal of characterizing inflammatory changes that result from sexual trauma. Pursuit of these aims will allow mentees to receive broad instruction on the design and management of clinical research protocols, collection and processing of biologic samples and execution of laboratory experiments involving state-of-the-art techniques in gene and protein expression and tissue imaging. Benchmarks for evaluating the progress of this K24 application will include the productivity of mentees measured by the number of abstracts, presentations and peer-reviewed publications as well as the number of career developments grants (NIH K- series, private foundation) that result from my patient oriented research program.

Public Health Relevance

This application for a K24 Midcareer Investigator Award will allow the principal investigator to spend 50% of his time and effort to mentor junior investigators in patient oriented research on HIV pathogenesis and treatment. The investigator will use his background in clinical medicine and basic science to introduce mentees to the processes involved in testing hypothesis driven research that spans the clinic and basic science laboratory.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Midcareer Investigator Award in Patient-Oriented Research (K24)
Project #
5K24HD080539-04
Application #
9249649
Study Section
AIDS Immunology and Pathogenesis Study Section (AIP)
Program Officer
Lorenzo, Eric
Project Start
2014-04-10
Project End
2019-03-31
Budget Start
2017-04-01
Budget End
2018-03-31
Support Year
4
Fiscal Year
2017
Total Cost
$161,487
Indirect Cost
$11,962
Name
Rhode Island Hospital
Department
Type
Independent Hospitals
DUNS #
075710996
City
Providence
State
RI
Country
United States
Zip Code
02903
Cheruiyot, Collins; Pataki, Zemplen; Williams, Robert et al. (2017) SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells. J Vis Exp :
Li, Ming; Lee, Kiho; Hsu, Min et al. (2017) Lactobacillus-derived extracellular vesicles enhance host immune responses against vancomycin-resistant enterococci. BMC Microbiol 17:66
Monnig, Mollie A; Kahler, Christopher W; Cioe, Patricia A et al. (2017) Markers of Microbial Translocation and Immune Activation Predict Cognitive Processing Speed in Heavy-Drinking Men Living with HIV. Microorganisms 5:
Jamwal, Rohitash; Topletz, Ariel R; Ramratnam, Bharat et al. (2017) Ultra-high performance liquid chromatography tandem mass-spectrometry for simple and simultaneous quantification of cannabinoids. J Chromatogr B Analyt Technol Biomed Life Sci 1048:10-18
Monnig, Mollie A; Kahler, Christopher W; Cioe, Patricia A et al. (2016) Alcohol use predicts elevation in inflammatory marker soluble CD14 in men living with HIV. AIDS Care 28:1434-40
Ahsan, Nagib; Rao, R Shyama Prasad; Gruppuso, Philip A et al. (2016) Targeted proteomics: Current status and future perspectives for quantification of food allergens. J Proteomics 143:15-23
Li, Ming; Ramratnam, Bharat (2016) Proteomic Characterization of Exosomes from HIV-1-Infected Cells. Methods Mol Biol 1354:311-26
Hughes, Brenna L; Dutt, Riana; Raker, Christina et al. (2016) The impact of pregnancy on anti-HIV activity of cervicovaginal secretions. Am J Obstet Gynecol 215:748.e1-748.e12
Sankapal, Soni; Gupta, Phalguni; Ratner, Deena et al. (2016) HIV Exposure to the Epithelia in Ectocervical and Colon Tissues Induces Inflammatory Cytokines Without Tight Junction Disruption. AIDS Res Hum Retroviruses 32:1054-1066
Li, Ming; Tucker, Lynne D; Asara, John M et al. (2016) Stem-loop binding protein is a multifaceted cellular regulator of HIV-1 replication. J Clin Invest 126:3117-29

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