: Funding is sought for a five year transitional training period for Dr. S. Manduchi to complete the move from Mathematics to Computational Biology/Bioinformatics. Dr. Manduchi has a strong training in Mathematics at the research level, a solid knowledge of Statistics, and an intermediate training in Computer Science. She has begun the transition into Computational Biology/Bioinformatics with a two year postdoc in the Biology Department at the University of Pennsylvania (Upenn), under professor Warren Ewens (funded by the National Science Foundation). During this postdoc she has split her time between the Computational Biology and Informatics Laboratory (CBIL) and working with Professor Ewens and others on several research projects. This resulted in two papers; one published, one accepted for publication. She has established several productive collaborations on various projects with members of PCBI (the Penn Center for Bioinformatics, of which CBIL is part) and its collaborators. Therefore it is desired to get funding to extend the stay for five years. The work plan for the five years is: 1) To continue working on the ongoing research projects on gene expression analysis, disease gene locating, developing genomics resources (databases and tools), and tissue-specific regulation. There will of course be many other unforseen research projects over the period. Computer, mathematical and statistical methods are becoming ubiquitous in all aspects of the health sciences and there will continue to be a great need for collaboration between researchers from different backgrounds in the Upenn's large biomedical complex. At least 60% working effort will be given to basic research of this type. 2) To attend courses in Biochemistry and Biology, as well as specific courses in Computer Science and Statistics that are relevant to Bioinformatics; roughly one course per semester. 3) To attend an average of one mini-course or workshop and two conferences per year in the held of Computational Biology/Bioinformatics. 4) To conduct a six month lab rotation in the laboratory of Dr. Klaus Kaestner (Dept. of Genetics, Upenn). 5) To continue participation in the PCBI reading group, in the organization of the two-semester Computational Biology course, and in the coordination of the Computational Biology/Bioinformatics Seminar series at Upenn.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Mentored Quantitative Research Career Development Award (K25)
Project #
5K25HG002296-02
Application #
6536484
Study Section
Ethical, Legal, Social Implications Review Committee (GNOM)
Program Officer
Graham, Bettie
Project Start
2001-05-11
Project End
2006-04-30
Budget Start
2002-06-01
Budget End
2003-04-30
Support Year
2
Fiscal Year
2002
Total Cost
$105,528
Indirect Cost
Name
University of Pennsylvania
Department
Genetics
Type
Schools of Medicine
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Hardy, Olga T; Hohmeier, Hans E; Becker, Thomas C et al. (2007) Functional genomics of the beta-cell: short-chain 3-hydroxyacyl-coenzyme A dehydrogenase regulates insulin secretion independent of K+ currents. Mol Endocrinol 21:765-73
Simmons, Craig A; Grant, Gregory R; Manduchi, Elisabetta et al. (2005) Spatial heterogeneity of endothelial phenotypes correlates with side-specific vulnerability to calcification in normal porcine aortic valves. Circ Res 96:792-9
Passerini, Anthony G; Shi, Congzhu; Francesco, Nadeene M et al. (2005) Regional determinants of arterial endothelial phenotype dominate the impact of gender or short-term exposure to a high-fat diet. Biochem Biophys Res Commun 332:142-8
Marciano, Paolo G; Brettschneider, Julia; Manduchi, Elisabetta et al. (2004) Neuron-specific mRNA complexity responses during hippocampal apoptosis after traumatic brain injury. J Neurosci 24:2866-76
Manduchi, E; Grant, G R; He, H et al. (2004) RAD and the RAD Study-Annotator: an approach to collection, organization and exchange of all relevant information for high-throughput gene expression studies. Bioinformatics 20:452-9
Passerini, Anthony G; Polacek, Denise C; Shi, Congzhu et al. (2004) Coexisting proinflammatory and antioxidative endothelial transcription profiles in a disturbed flow region of the adult porcine aorta. Proc Natl Acad Sci U S A 101:2482-7
Grant, G R; Manduchi, E; Pizarro, A et al. (2003) Maintaining data integrity in microarray data management. Biotechnol Bioeng 84:795-800
Polacek, Denise C; Passerini, Anthony G; Shi, Congzhu et al. (2003) Fidelity and enhanced sensitivity of differential transcription profiles following linear amplification of nanogram amounts of endothelial mRNA. Physiol Genomics 13:147-56
Manduchi, Elisabetta; Scearce, L Marie; Brestelli, John E et al. (2002) Comparison of different labeling methods for two-channel high-density microarray experiments. Physiol Genomics 10:169-79