This is a resubmission of an application for a career development award for Dr. Guy M. Genin in collaboration with Dr. Elliot L Elson. Dr. Genin is a Mechanical Engineering professor at Washington University in St. Louis (WU) who aims to shift his career towards quantitative biology. Dr. Elson heads a vigorous research program at WU aimed at developing viable bioartificial tissues, and is supported by the NIH. The focus of the 5-year research and training program is developing expertise in applying solid mechanics concepts to study active force regulation by cells in bioartificial tissues. Initially, Dr. Genin plans an intensive program of coursework and formal didactic study, coupled with laboratory work. After two years of study, the applicant plans a sabbatical year in Dr. Elson's lab, collaborating on mechanical characterization of idealized tissue constructs. In the final two years, the applicant would continue to collaborate on studies relating macroscopic tissue behavior and microscopic cellular behavior. By the end of the research and training plan, Dr. Genin would be situated to become a leading partner in interdisciplinary studies of cell and tissue engineering at WU, and supported by the NIH. The research plan aims to quantify the relationship between cellular behavior and tissue behavior in idealized tissue constructs. This is vital for developing artificial tissues whose mechanical properties mimic those of the healthy tissues they replace, and for determining the mechanical response of cells in vivo. The primary analytical tool with which the applicant will begin these studies is an improved constitutive model for bioartificial tissues (Aim 1). The work will also require careful study of remodeled collagen (Aim 2.) The applicant will proceed to study progressively more complicated tissue constructs: first, reconstituted collagen populated with cardiac fibroblasts (Aim 3), and then a similar tissue populated with cardiac fibroblasts and cardiomyocytes (Aim 4.)

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Mentored Quantitative Research Career Development Award (K25)
Project #
5K25HL079165-05
Application #
7825471
Study Section
Special Emphasis Panel (ZHL1-CSR-M (O1))
Program Officer
Roltsch, Mark
Project Start
2006-06-01
Project End
2012-05-31
Budget Start
2010-06-01
Budget End
2012-05-31
Support Year
5
Fiscal Year
2010
Total Cost
$140,671
Indirect Cost
Name
Washington University
Department
Engineering (All Types)
Type
Schools of Engineering
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Embar, Anand; Dolbow, John; Fried, Eliot (2013) Microdomain evolution on giant unilamellar vesicles. Biomech Model Mechanobiol 12:597-615
Namani, R; Feng, Y; Okamoto, R J et al. (2012) Elastic characterization of transversely isotropic soft materials by dynamic shear and asymmetric indentation. J Biomech Eng 134:061004
Pryse, Kenneth M; Rong, Xi; Whisler, Jordan A et al. (2012) Confidence intervals for concentration and brightness from fluorescence fluctuation measurements. Biophys J 103:898-906
Lee, Sheng-Lin; Nekouzadeh, Ali; Butler, Boyd et al. (2012) Physically-induced cytoskeleton remodeling of cells in three-dimensional culture. PLoS One 7:e45512
Liu, Y X; Thomopoulos, S; Birman, V et al. (2012) Bi-material attachment through a compliant interfacial system at the tendon-to-bone insertion site. Mech Mater 44:
Alexander, Benjamin; Daulton, Tyrone L; Genin, Guy M et al. (2012) The nanometre-scale physiology of bone: steric modelling and scanning transmission electron microscopy of collagen-mineral structure. J R Soc Interface 9:1774-86
Bayly, Philip V; Clayton, Erik H; Genin, Guy M (2012) Quantitative imaging methods for the development and validation of brain biomechanics models. Annu Rev Biomed Eng 14:369-96
Nekouzadeh, Ali; Genin, Guy M (2011) Quantification of fibre polymerization through Fourier space image analysis. Proc Math Phys Eng Sci 467:2310-2329
Liu, Yanxin; Birman, Victor; Chen, Changqing et al. (2011) Mechanisms of Bimaterial Attachment at the Interface of Tendon to Bone. J Eng Mater Technol 133:
Thomopoulos, Stavros; Das, Rosalina; Birman, Victor et al. (2011) Fibrocartilage tissue engineering: the role of the stress environment on cell morphology and matrix expression. Tissue Eng Part A 17:1039-53

Showing the most recent 10 out of 20 publications