Mitochondrial quality in skeletal muscle progressively declines with advancing age, leading to tissue dysfunction and disease. Several lines of evidence suggest poor mitochondrial quality in skeletal muscle of old animals and humans is due in large part to an impaired or insufficient capacity to degrade damaged/dysfunctional mitochondria via mitophagy. Exercise promotes mitochondrial quality leading to healthy aging but the underlying mechanisms and how they differ with age is not well defined, particularly in regards to mitophagy, restraining development of effective interventions. Building upon my previous work demonstrating that exercise does indeed promote mitophagy in skeletal muscle but only of a small fraction of the total mitochondrial reticulum, I show here evidence of an Ampk-dependent mechanism that may distinguish damaged vs. healthy regions of the mitochondrial reticulum that is lost with age. Additionally, I show that key downstream mitophagy-related factors that are recruited to mitochondria in skeletal muscle with exercise are required for mitochondrial quality and healthy aging in d. melanogaster. The proposed research tests the hypothesis that recognition of damaged regions of the mitochondrial reticulum in response to exercise is impaired in skeletal muscle of old mice, blunting local recruitment of key mitophagy proteins, leading to poor mitochondrial quality. These studies will provide insight into novel regulation of skeletal muscle mitophagy in response to exercise and lay a foundation for the development of targeted interventions to promote mitochondrial quality in skeletal muscle for improved tissue function and healthy aging. During the mentored phase, I will employ state-of-the-art two-photon microscopy to perform intravital and ex vivo fluorescent lifetime microscopy of Ampk activity on mitochondria in skeletal muscle of young and old mice to determine the age-dependent, localized response of Ampk to sustained contraction and mitochondrial damage. Also, I will continue my professional and scientific development in preparation for the independent phase with continuous guidance from my mentoring committee. During the independent phase, I will employ co-somatic gene transfer in skeletal muscle of young and old wild-type mice as well as skeletal muscle-specific, conditional Ulk1 knock-out mice to determine the age-dependent regulation for the recruitment of downstream mitophagy-related factors to mitochondria in skeletal muscle in response to exercise. Also, I will develop novel phospho-mimetic constructs to constitutively activate or inhibit mitophagy-related factors Atg9 and Atg2 in young and old mouse skeletal muscle and investigate their necessity and sufficiency for the breakdown of mitochondrial proteins and maintenance of mitochondrial quality, via state-of-the-art high resolution proteomic and confocal imaging approaches. Collectively, these studies and career development activities will foster my continued scientific and professional training, leading to a successful independent, academic research program.

Public Health Relevance

Skeletal muscle mitochondrial quality declines with age due to impaired or insufficient degradation of damaged/dysfunctional mitochondria, contributing to the age-dependent decline in tissue function, and thus, is a barrier to healthy aging. My project will give important insight into a novel mechanism for how damaged/dysfunctional regions of the mitochondrial reticulum are recognized and the capacity for this mechanism in skeletal muscle of old mice, as well as how downstream mitophagy-related factors are regulated by exercise in young vs old skeletal muscle. Elucidation of these important mechanisms will expand the field of mitochondrial quality control within geroscience and provide novel insight into potential mitochondria modulators for the development of targeted interventions that promote mitochondrial quality so to remove barriers to healthy aging.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Career Transition Award (K99)
Project #
1K99AG057825-01A1
Application #
9599150
Study Section
Neuroscience of Aging Review Committee (NIA)
Program Officer
Fridell, Yih-Woei
Project Start
2018-08-15
Project End
2020-07-31
Budget Start
2018-08-15
Budget End
2019-07-31
Support Year
1
Fiscal Year
2018
Total Cost
Indirect Cost
Name
University of Virginia
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
065391526
City
Charlottesville
State
VA
Country
United States
Zip Code
22904