This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study will determine if the energy expenditure of daily living can be precisely estimated. Energy expenditure from activity can be broken down into two groups: exercise-related thermogenesis and non-exercise activity thermogenesis (NEAT). NEAT is the energy expended during daily activities such as occupation, sitting, standing, and fidgeting. Due to the variety of the components of NEAT, it is challenging to measure. In this study a Physical Activity Measuring System (PAMS) will be used to measure NEAT. PAMS consists of small devices that can determine body position and motion. Subjects will wear PAMS in a free-living environment and in a lab setting where their energy expenditure will be measured during sitting, standing, and the transition between sitting and standing. The energy expenditure information obtained from PAMS will be compared to information obtained the lab testing. Differences in energy expenditure, walking velocity, walking movement, and minutes spent walking will be compared between lean and obese subjects. This comparison will give insight into the differences in energy balance between lean and obese humans.
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