This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.An investigation of whether tissue contents of DCI (D-chiro-inositol) and DCI-IPG (inositolphosphoglycan) are decreased in muscle and ovary obtained from women with polycystic ovary syndrome (PCOS) as compared with age and weight-matched normal women.PCOS is a poorly understood disorder that affects 6-10% of women of reproductive age. PCOS is characterized by an excessive secretion of masculine hormones and lack of ovulation and is the leading cause of infertility in the United States. Women with PCOS are also at high risk for developing type 2 diabetes, presumably due to the insulin resistance that accompanies the syndrome. This study is designed to determine if the tissue ratio of total DCI to MYO and the ratio DCI-IPG to MYO-IPG in muscle and ovary of women with PCOS are decreased compared to those in the muscle and ovary of age and weight-matched normal women. There will be three study sessions taking place at the General Clinical Research Center. The study will take place within two weeks of gynecological surgery. On the first day, blood pressure, height, weight, and body measurements will be done. A medical and menstrual history will be taken. A urine pregnancy test will be done. A urine kit with directions for a 24 hour urine sample will be given to the subject. The sample will be returned to the clinic the next morning. A fast will begin at 8:00 pm. DCI intake can come from a diet high in vegetables and fruits. A dietician will discuss a balanced, well mixed diet with the subjects which they must follow for at least three days before the start of the study.On day two, the subjects will return to the clinic after a 12 hour fast. Blood will be drawn through a heparin lock which is a plastic catheter inserted into a vein which allows blood samples to be obtained periodically without repeated sticks. An oral glucose tolerance test will be done where the patient drinks glucose and blood tests are done over the next few hours. A transvaginal ultrasound will be done and instructions for another fast starting at 8:00 pm will be given.On the third day, after the 12 hour fast, an insulin sensitivity test will be done. This test uses an intravenous infusion of glucose and several blood samples are taken through the heparin lock. At the time of the scheduled surgery, one ovary and biopsies taken from the abnormal muscle will be used for testing in this research.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
General Clinical Research Centers Program (M01)
Project #
2M01RR000065-45
Application #
7604996
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Project Start
2006-12-20
Project End
2007-11-30
Budget Start
2006-12-20
Budget End
2007-11-30
Support Year
45
Fiscal Year
2007
Total Cost
$2,107
Indirect Cost
Name
Virginia Commonwealth University
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
105300446
City
Richmond
State
VA
Country
United States
Zip Code
23298
Holkova, Beata; Yazbeck, Victor; Kmieciak, Maciej et al. (2017) A phase 1 study of bortezomib and romidepsin in patients with chronic lymphocytic leukemia/small lymphocytic lymphoma, indolent B-cell lymphoma, peripheral T-cell lymphoma, or cutaneous T-cell lymphoma. Leuk Lymphoma 58:1349-1357
Corey, Kathleen E; Vuppalanchi, Raj; Vos, Miriam et al. (2015) Improvement in liver histology is associated with reduction in dyslipidemia in children with nonalcoholic fatty liver disease. J Pediatr Gastroenterol Nutr 60:360-7
Eaton, J E; Juran, B D; Atkinson, E J et al. (2015) A comprehensive assessment of environmental exposures among 1000 North American patients with primary sclerosing cholangitis, with and without inflammatory bowel disease. Aliment Pharmacol Ther 41:980-90
Worthington Jr, Everett L; Berry, Jack W; Hook, Joshua N et al. (2015) Forgiveness-reconciliation and communication-conflict-resolution interventions versus retested controls in early married couples. J Couns Psychol 62:14-27
Holkova, Beata; Kmieciak, Maciej; Perkins, E Brent et al. (2014) Phase I trial of bortezomib (PS-341; NSC 681239) and ""nonhybrid"" (bolus) infusion schedule of alvocidib (flavopiridol; NSC 649890) in patients with recurrent or refractory indolent B-cell neoplasms. Clin Cancer Res 20:5652-62
Noureddin, Mazen; Yates, Katherine P; Vaughn, Ivana A et al. (2013) Clinical and histological determinants of nonalcoholic steatohepatitis and advanced fibrosis in elderly patients. Hepatology 58:1644-54
Lo, D J; Anderson, D J; Weaver, T A et al. (2013) Belatacept and sirolimus prolong nonhuman primate renal allograft survival without a requirement for memory T cell depletion. Am J Transplant 13:320-8
Poklepovic, Andrew; Youssefian, Leena E; Youseffian, Leena et al. (2013) Phase I trial of bortezomib and dacarbazine in melanoma and soft tissue sarcoma. Invest New Drugs 31:937-42
Holkova, Beata; Supko, Jeffrey G; Ames, Matthew M et al. (2013) A phase I trial of vorinostat and alvocidib in patients with relapsed, refractory, or poor prognosis acute leukemia, or refractory anemia with excess blasts-2. Clin Cancer Res 19:1873-83
Lo, D J; Farris, A B; Song, M et al. (2013) Inhibition of ?v?6 promotes acute renal allograft rejection in nonhuman primates. Am J Transplant 13:3085-93

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