1. Separate M. leprae baccili from infected armadillo tissues and distribute the purified bacilli to investigators upon request. 2. Purify and supply phenolic glycolipid I and dimycocerosylphthiocerol from M. leprae-free supernatant material derived from homogenization of infected armadillo tissue. 3. Synthesize and provide the Neoglycoconjugates based on phenolic glycolipid I for the purpose of the serological diagnosis of leprosy. 4. Purify and provide the native lipoarabinomannan and lipomannan of M. leprae and M. tuberculosis. 5. Isolate the peptidoglycan-associated proteins of M. leprae. 6. Isolate, purify and characterize other native protein antigens and sub- cellular entities from M. leprae.

Project Start
1990-07-01
Project End
1995-06-30
Budget Start
1994-03-28
Budget End
1995-06-30
Support Year
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Colorado State University-Fort Collins
Department
Public Health & Prev Medicine
Type
Schools of Veterinary Medicine
DUNS #
112617480
City
Fort Collins
State
CO
Country
United States
Zip Code
80523
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Roach, T I; Barton, C H; Chatterjee, D et al. (1993) Macrophage activation: lipoarabinomannan from avirulent and virulent strains of Mycobacterium tuberculosis differentially induces the early genes c-fos, KC, JE, and tumor necrosis factor-alpha. J Immunol 150:1886-96
Daffe, M; McNeil, M; Brennan, P J (1993) Major structural features of the cell wall arabinogalactans of Mycobacterium, Rhodococcus, and Nocardia spp. Carbohydr Res 249:383-98
Hines 2nd, M E; Jaynes, J M; Barker, S A et al. (1993) Isolation and partial characterization of glycolipid fractions from Mycobacterium avium serovar 2 (Mycobacterium paratuberculosis 18) that inhibit activated macrophages. Infect Immun 61:1-7

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