Abstract

1. Maintain closed, quarantine protected conventional colony of circa 7 to 8000 inbred (genetically defined) mice which includes BALB/c sublines, BALB/c congenic strains and other relevant inbred strains. 2. Production breeding of selected inbred and congenic mice for inductiontransplantation experiments. 3. Development of congenic strains of mice carrying specific markers designated by Project Officer. This includes monitoring the introgressive backcrossing. This involves the use of isozyme and restriction. fragment polymorphism marker identification technology. 4. Maintain and breed according to defined protocols a colony of wild mice including near and distant relatives of M. musculus domesticus. 5. Carry out plasmacytoma induction experiments which includes i.p. injection of pristane, treatment with pharmacological agents (indomethacin, etc.) and various transforming retroviruses. 6. Transplant, freeze and recover viable plasmacytomas and related tumors. 7. Respond to and send out transplantable tumors, pedigreed breeding mice and DNA samples from mice in the colony to responsible investigators in the US and abroad. 8. Isolate high molecular weight DNA from mice and from surgically removed tissues.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Division of Cancer Biology And Diagnosis (NCI)
Type
Research and Development Contracts (N01)
Project #
N01CB071085-010
Application #
3606038
Study Section
Project Start
1987-02-01
Project End
1992-01-31
Budget Start
1991-03-01
Budget End
1992-01-31
Support Year
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Hazleton Government Services Division
Department
Type
DUNS #
City
Rockville
State
MD
Country
United States
Zip Code
20850

  Publications

Podlaha, Ondrej; Webb, David M; Tucker, Priscilla K et al. (2005) Positive selection for indel substitutions in the rodent sperm protein catsper1. Mol Biol Evol 22:1845-52
Tucker, Priscilla K; Adkins, Ronald M; Rest, Joshua S (2003) Differential rates of evolution for the ZFY-related zinc finger genes, Zfy, Zfx, and Zfa in the mouse genus Mus. Mol Biol Evol 20:999-1005
Lundrigan, Barbara L; Jansa, Sharon A; Tucker, Priscilla K (2002) Phylogenetic relationships in the genus mus, based on paternally, maternally, and biparentally inherited characters. Syst Biol 51:410-31
Lundrigan, B L; Tucker, P K (1994) Tracing paternal ancestry in mice, using the Y-linked, sex-determining locus, Sry. Mol Biol Evol 11:483-92
Nagamine, C M (1994) The testis-determining gene, SRY, exists in multiple copies in Old World rodents. Genet Res 64:151-9
Tucker, P K; Lundrigan, B (1993) Comparative studies of mammalian Y chromosome. Methods Enzymol 224:517-25
Garagna, S; Redi, C A; Capanna, E et al. (1993) Genome distribution, chromosomal allocation, and organization of the major and minor satellite DNAs in 11 species and subspecies of the genus Mus. Cytogenet Cell Genet 64:247-55
Ong, G L; Baker, A E; Mattes, M J (1992) Analysis of high complement levels in Mus hortulanus and BUB mice. J Immunol Methods 154:37-45
Chin, H; Krall, M; Kim, H L et al. (1992) The gene for the alpha 1 subunit of the skeletal muscle dihydropyridine-sensitive calcium channel (Cchl1a3) maps to mouse chromosome 1. Genomics 14:1089-91
Byrd, L; Grossmann, M; Potter, M et al. (1991) Chronic multifocal osteomyelitis, a new recessive mutation on chromosome 18 of the mouse. Genomics 11:794-8

Showing the most recent 10 out of 11 publications