To develop alternative methods for the testing of chemicals for toxicity, mutagenicity, and/or carcinogenicity in order to increase test sensitivity, decrease time requirements, and reduce the number of animals required for chemical safety evaluation. The selection, development, and evaluation of transgenic mouse model systems has the following three main goals: (1) screen large numbers of chemicals (mutagens and non-mutagens) in a rapid and efficient manner, (2) analyze and compare mutagenic (and non-mutagenic events affecting cell function) and carcinogenic events at the chromosomal and/or molecular level within the same protocol, and (3) develop cultured transgenic cell lines from normal tissues and spontaneous and chemically induced site specific tumors for detailed analysis.
| Tice, R R; Furedi-Machacek, M; Satterfield, D et al. (1998) Measurement of micronucleated erythrocytes and DNA damage during chronic ingestion of phenolphthalein in transgenic female mice heterozygous for the p53 gene. Environ Mol Mutagen 31:113-24 |
| Tice, R R; Nylander-French, L A; French, J E (1997) Absence of systemic in vivo genotoxicity after dermal exposure to ethyl acrylate and tripropylene glycol diacrylate in Tg.AC (v-Ha-ras) mice. Environ Mol Mutagen 29:240-9 |
| Dunnick, J K; Hardisty, J F; Herbert, R A et al. (1997) Phenolphthalein induces thymic lymphomas accompanied by loss of the p53 wild type allele in heterozygous p53-deficient (+/-) mice. Toxicol Pathol 25:533-40 |
| Tennant, R W; French, J E; Spalding, J W (1995) Identifying chemical carcinogens and assessing potential risk in short-term bioassays using transgenic mouse models. Environ Health Perspect 103:942-50 |
