Abstract

The specific objective of this study is to develop monoclonal antibodies to be used in the study of limb development. The Contractor shall: (1) Organize a monoclonal antibody bank to provide investigators with published monoclonal antibodies that recognize various macromolecular components in the developing limb. (2) Develop well characterized new monoclonal antibodies for inclusion in the antibody bank to the macromolecules, Chondronectin and Fibronectin and where possible also to other macromolecules in the developing limb. For both the published and the newly developed monoclonal antibodies, the contractor must be willing to comply to the species specificity of antibodies required by investigators since most limb development studies are currently being done in only a few animal models. (3) Distribute monoclonal antibodies to limb development investigators for a small fee as directed by the Project Officer. Obtain from investigators and transmit to Project Officer, reports and publications of research in which the monoclonal antibodies have been employed.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research and Development Contracts (N01)
Project #
N01HD062915-001
Application #
3652182
Study Section
Project Start
1986-02-01
Project End
1991-01-14
Budget Start
1987-02-06
Budget End
1988-02-01
Support Year
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Chang, Chia-Hao; Yang, Wen-Kai; Lin, Chia-Hao et al. (2016) FXYD11 mediated modulation of Na(+)/K(+)-ATPase activity in gills of the brackish medaka (Oryzias dancena) when transferred to hypoosmotic or hyperosmotic environments. Comp Biochem Physiol A Mol Integr Physiol 194:19-26
Yang, Sheng-Hui; Kang, Chao-Kai; Hu, Yau-Chung et al. (2015) Comparisons of two types of teleostean pseudobranchs, silver moony (Monodactylus argenteus) and tilapia (Oreochromis mossambicus), with salinity-dependent morphology and ion transporter expression. J Comp Physiol B 185:677-93
Kang, Chao-Kai; Yang, Shu-Yuan; Lin, Shang-Tao et al. (2015) The inner opercular membrane of the euryhaline teleost: a useful surrogate model for comparisons of different characteristics of ionocytes between seawater- and freshwater-acclimated medaka. Histochem Cell Biol 143:69-81
Tang, Cheng-Hao; Leu, Ming-Yih; Yang, Wen-Kai et al. (2014) Exploration of the mechanisms of protein quality control and osmoregulation in gills of Chromis viridis in response to reduced salinity. Fish Physiol Biochem 40:1533-46
Chang, Chun-Yu; Tang, Cheng-Hao; Hsin, Yi-Hong et al. (2014) FXYD2c plays a potential role in modulating Na(+)/K (+)-ATPase activity in HK-2 cells upon hypertonic challenge. J Membr Biol 247:93-105
Yang, Sheng-Hui; Kang, Chao-Kai; Kung, Hsiu-Ni et al. (2014) The lamellae-free-type pseudobranch of the euryhaline milkfish (Chanos chanos) is a Na(+), K(+)-ATPase-abundant organ involved in hypoosmoregulation. Comp Biochem Physiol A Mol Integr Physiol 170:15-25
Kang, Chao-Kai; Yang, Wen-Kai; Lin, Shang-Tao et al. (2013) The acute and regulatory phases of time-course changes in gill mitochondrion-rich cells of seawater-acclimated medaka (Oryzias dancena) when exposed to hypoosmotic environments. Comp Biochem Physiol A Mol Integr Physiol 164:181-91
Yang, Wen-Kai; Kang, Chao-Kai; Chang, Chia-Hao et al. (2013) Expression profiles of branchial FXYD proteins in the brackish medaka Oryzias dancena: a potential saltwater fish model for studies of osmoregulation. PLoS One 8:e55470
Kang, Chao-Kai; Liu, Fu-Chen; Chang, Wen-Been et al. (2012) Effects of low environmental salinity on the cellular profiles and expression of Na+, K+-ATPase and Na+, K+, 2Cl- cotransporter 1 of branchial mitochondrion-rich cells in the juvenile marine fish Monodactylus argenteus. Fish Physiol Biochem 38:665-78
Tang, Cheng-Hao; Lai, Dong-Yang; Lee, Tsung-Han (2012) Effects of salinity acclimation on Na(+)/K(+)-ATPase responses and FXYD11 expression in the gills and kidneys of the Japanese eel (Anguilla japonica). Comp Biochem Physiol A Mol Integr Physiol 163:302-10

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