Abstract

The objectives of this contract are 1) to purify, isolate and characterize gonadal polypeptides (particularly oocyte maturation inhibiting factor(s), follicular regulatory protein(s) and other growth factors present in porcine follicular fluid) and 2) to prepare partially purified polypeptide factors for distribution by the Government after independent verification of the level of biological activity has been obtained.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research and Development Contracts (N01)
Project #
N01HD062944-003
Application #
3652392
Study Section
Project Start
1986-09-30
Project End
1989-09-29
Budget Start
1988-06-10
Budget End
1989-09-29
Support Year
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Salk Institute for Biological Studies
Department
Type
DUNS #
005436803
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

DePaolo, L V (1991) Hypersecretion of follicle-stimulating hormone (FSH) after ovariectomy of hypophysectomized, pituitary-grafted rats: implications for local regulatory control of FSH. Endocrinology 128:1731-40
Shimasaki, S; Shimonaka, M; Ui, M et al. (1990) Structural characterization of a follicle-stimulating hormone action inhibitor in porcine ovarian follicular fluid. Its identification as the insulin-like growth factor-binding protein. J Biol Chem 265:2198-202
Bicsak, T A; Shimonaka, M; Malkowski, M et al. (1990) Insulin-like growth factor-binding protein (IGF-BP) inhibition of granulosa cell function: effect on cyclic adenosine 3',5'-monophosphate, deoxyribonucleic acid synthesis, and comparison with the effect of an IGF-I antibody. Endocrinology 126:2184-9
Ling, N; DePaolo, L V; Bicsak, T A et al. (1990) Novel ovarian regulatory peptides: inhibin, activin, and follistatin. Clin Obstet Gynecol 33:690-702
Bicsak, T A; Nakatani, A; Shimonaka, M et al. (1990) Insulin-like growth factor binding protein measurement: sodium dodecyl sulfate-stable complexes with insulin-like growth factor in serum prevent accurate assessment of total binding protein content by ligand blotting. Anal Biochem 191:75-9
Shimasaki, S; Koba, A; Mercado, M et al. (1989) Complementary DNA structure of the high molecular weight rat insulin-like growth factor binding protein (IGF-BP3) and tissue distribution of its mRNA. Biochem Biophys Res Commun 165:907-12
Ui, M; Shimonaka, M; Shimasaki, S et al. (1989) An insulin-like growth factor-binding protein in ovarian follicular fluid blocks follicle-stimulating hormone-stimulated steroid production by ovarian granulosa cells. Endocrinology 125:912-6
Shimonaka, M; Schroeder, R; Shimasaki, S et al. (1989) Identification of a novel binding protein for insulin-like growth factors in adult rat serum. Biochem Biophys Res Commun 165:189-95
Ling, N; Ueno, N; Ying, S Y et al. (1988) Inhibins and activins. Vitam Horm 44:1-46
Ueno, N; Ling, N; Ying, S Y et al. (1987) Isolation and partial characterization of follistatin: a single-chain Mr 35,000 monomeric protein that inhibits the release of follicle-stimulating hormone. Proc Natl Acad Sci U S A 84:8282-6

Showing the most recent 10 out of 12 publications