We will develop a novel system for detecting chemicals which induce peroxisome proliferation. This system will consist of a human cell line which has been transfected with specific receptor and reporter gene constructs. Cells will be transfected with receptors for peroxisome proliferators derived from different species. The reporter gene (cDNA) is human cytochrome P450 which catalyzes formation of a fluorescent product. The reporter cDNA is coupled to a promoter linked to a peroxisome proliferator responsive element. The resulting assay system should be specific for peroxisome proliferators and easily amenable to automation for screening large numbers of chemicals.

Project Start
1993-09-30
Project End
1995-09-29
Budget Start
1995-04-19
Budget End
1996-09-29
Support Year
Fiscal Year
1995
Total Cost
Indirect Cost
Name
Gentest Corporation
Department
Type
DUNS #
City
Woburn
State
MA
Country
United States
Zip Code
01801