Abstract

The objective is to study the growth and function of cultured human endothelial cells (EC) isolated from diverse vascular sites in different aged donors of both sexes. Emphasis will be placed on examining proliferative potential, selected metabolic activities, certain transport functions, and membrane properties related to hemostatic function. Vascular tissue will be obtained from different sites in brain-dead, heart-beating cadavers, and large vessel and capillary EC will be isolated. Primary, secondary and cloned EC cultures will be generated and distributed to the component projects. The following goals will be pursued: determine the in vitro lifespan of EC; examine cytogenetic properties of EC; investigate the roles of and cooperative interactions between various culture components as they affect human EC growth in Phase II and Phase III cells from young donors and Phase II cells from older donors; measure the metabolites of arachidonic acid synthesized by human EC; characterize and quantify human EC interaction with Factor VIII-related antigen and other coagulation components; and measure vesicular ingestion capacity and insulin binding in human EC and examine the effect of different collagen substrata on these EC functions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG004861-05
Application #
3090930
Study Section
Aging Review Committee (AGE)
Project Start
1985-12-01
Project End
1991-11-30
Budget Start
1989-12-01
Budget End
1991-11-30
Support Year
5
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
Wistar Institute
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Sorger, T; Friday, N; Yang, L D et al. (1995) Heparin and the phenotype of adult human vascular smooth muscle cells. In Vitro Cell Dev Biol Anim 31:671-83
Rajagopalan, V; Essex, D W; Shapiro, S S et al. (1992) Tumor necrosis factor-alpha modulation of glycoprotein Ib alpha expression in human endothelial and erythroleukemia cells. Blood 80:153-61
Johnson, T E; Umbenhauer, D R; Hill, R et al. (1992) Karyotypic and phenotypic changes during in vitro aging of human endothelial cells. J Cell Physiol 150:17-27
Wojenski, C M; Schick, P K (1991) Differences in thromboxane A2 synthesis by megakaryocytes and platelets. J Lab Clin Med 118:370-6
Ando, J; Albelda, S M; Levine, E M (1991) Culture of human adult endothelial cells on liquid-liquid interfaces: a new approach to the study of cell-matrix interactions. In Vitro Cell Dev Biol 27A:525-32
Konkle, B A; Shapiro, S S; Asch, A S et al. (1990) Cytokine-enhanced expression of glycoprotein Ib alpha in human endothelium. J Biol Chem 265:19833-8
Mueller, S N; Thomas, K A; Di Salvo, J et al. (1989) Stabilization by heparin of acidic fibroblast growth factor mitogenicity for human endothelial cells in vitro. J Cell Physiol 140:439-48
Rupnick, M A; Stokes, C L; Williams, S K et al. (1988) Quantitative analysis of random motility of human microvessel endothelial cells using a linear under-agarose assay. Lab Invest 59:363-72
Ingerman-Wojenski, C M; Silver, M J; Mueller, S N et al. (1988) Prostacyclin synthesis by endothelial cells from human umbilical veins: effect of cumulative population doublings. Prostaglandins 36:127-37
Sprandio, J D; Shapiro, S S; Thiagarajan, P et al. (1988) Cultured human umbilical vein endothelial cells contain a membrane glycoprotein immunologically related to platelet glycoprotein Ib. Blood 71:234-7

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