The Transgenic Mouse Core is a centralized facility designed to provide the techniques required by the individual projects for producing and maintaining transgenic mice. This facility will efficiently handle exacting techniques, such as microinjection of one cell embryos and the characterization of transgenic mice. The core will perform the following services: i) The core will produce transgenic mice using DNA submitted by each of the project investigators; ii) Transgenic mice will be identified by polymerase chain reaction (PCR) amplification procedures; iii) Transgenic founders will be further characterized by Southern analysis to determine the number of integration sites and the copy number of integrated transgenes; iv) Appropriate mice will be bred to maintain stocks and specific matings will be performed to establish transgenic lines homozygous for the transgene, as needed; v) The Transgenic Mouse Core will also be responsible for maintaining and housing mice for aging studies; and vi) Transgenic lines will be maintained through embryo cryopreservation. As a long-range goal the Core will establish techniques for obtaining and culturing embryonic stem (ES) cells. These cells will be available to generate transgenic mice via homologous recombination.
| Yang, F; Ghio, A J; Herbert, D C et al. (2000) Pulmonary expression of the human haptoglobin gene. Am J Respir Cell Mol Biol 23:277-82 |
| Yang, F; Coalson, J J; Bobb, H H et al. (1999) Resistance of hypotransferrinemic mice to hyperoxia-induced lung injury. Am J Physiol 277:L1214-23 |
| Reynolds, M D; Johnston, J M; Dodge, H H et al. (1999) Small head size is related to low Mini-Mental State Examination scores in a community sample of nondemented older adults. Neurology 53:228-9 |
| Ganguli, M; Lytle, M E; Reynolds, M D et al. (1998) Random versus volunteer selection for a community-based study. J Gerontol A Biol Sci Med Sci 53:M39-46 |
| Yang, F; Friedrichs, W E; Coalson, J J (1997) Regulation of transferrin gene expression during lung development and injury. Am J Physiol 273:L417-26 |
| Barnum-Huckins, K M; Martinez, A O; Rivera, E V et al. (1997) A comparison of the suppression of human transferrin synthesis by lead and lipopolysaccharide. Toxicology 118:11-22 |
| Bowman, B H; Yang, F; Buchanan, J M et al. (1996) Human APOE protein localized in brains of transgenic mice. Neurosci Lett 219:57-9 |
| Adrian, G S; Seto, E; Fischbach, K S et al. (1996) YY1 and Sp1 transcription factors bind the human transferrin gene in an age-related manner. J Gerontol A Biol Sci Med Sci 51:B66-75 |
| Yang, F; Friedrichs, W E; deGraffenried, L et al. (1996) Cellular expression of ceruloplasmin in baboon and mouse lung during development and inflammation. Am J Respir Cell Mol Biol 14:161-9 |
| Friedrichs, W E; Navarijo-Ashbaugh, A L; Bowman, B H et al. (1995) Expression and inflammatory regulation of haptoglobin gene in adipocytes. Biochem Biophys Res Commun 209:250-6 |
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