Lipids are essential cellular components and act as mediators in cell signaling. The function of lipids is determined by their structures. Dysregulated lipid metabolism is associated with a variety of pathological conditions, including Type 1 Diabetes (T1D). The levels of lipids cannot be predicted by the transcripts or proteins. To understand more about the role of lipids in the development of T1D, an important first step is to directly measure the temporal changes of lipids in an organism that shares similar physiology with human T1D. Built on our technological advancements in comprehensive global lipidomics, targeted lipidomic analysis of lipid mediators, isobaric labeling-based multiplexed analysis of intact glycolipids and determination of C=C unsaturation in intact lipids, in this project, we aim to profile the longitudinal changes of the nonobese diabetic (NOD) mouse lipidome during different stages of T1D development by accomplishing the following: 1) in-depth profile and accurately quantify the major lipids in T1D-pertinent tissues using global lipidomics; 2) use advanced technologies to improve structural resolution, accurately identify and quantify glycolipids and broadly profile bioactive lipid mediators; and 3) compare lipid expression profiles in different tissues and characterize lipidome remodeling during different stages of T1D development. Successfully accomplishing these goals will not only provide invaluable resource to the research community interested in the role of lipids in the development of T1D, but it will also provide better practices for conducting lipidomics research in general.

Public Health Relevance

Lipids are a class of biomolecules that are not only a major component of cellular membranes, but they also function as cell signaling mediators. Dysregulated lipid metabolism is associated with Type 1 Diabetes (T1D). Quantitative profiling of lipids in nonobese diabetic mouse ? a widely used model organism that shares similar physiology with human T1D, is an important step toward understanding the role of lipids in human T1D development.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK123499-02
Application #
10094214
Study Section
Enabling Bioanalytical and Imaging Technologies Study Section (EBIT)
Program Officer
Castle, Arthur
Project Start
2020-02-01
Project End
2024-01-31
Budget Start
2021-02-01
Budget End
2022-01-31
Support Year
2
Fiscal Year
2021
Total Cost
Indirect Cost
Name
University of North Carolina Greensboro
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
616152567
City
Greensboro
State
NC
Country
United States
Zip Code
27402