The neuropathology Core will provide, to the researchers of the Program Project, brain tissue and diagnostic neuropathology evaluations on Alzheimer's disease (AD) patients and controls, from subjects who have been closely followed in the Clinical Core with neurological and neuropsychological examinations. Brain tissue with minimal post-mortem intervals will be obtained and preserved so as to fill the needs of the three Program Project investigators. The abundant brain tissue received by the Rush Brain Bank from the Rush Alzheimer's Disease Center (RADC) and the establishment of new sources of control tissue, the Religious Order Study (ROS) and the RADC control program will allow the Neuropathology Core to fulfill the needs of the investigators. Each project will study a series of clinically defined brains': 8 controls without dementia and 24 AD patients divided equally between three groups; mild, moderate and severe cognitive impairment. One project will use fixed basal forebrain tissue and snap frozen cortical tissue, for immunohistochemical and in situ hybridization studies. Other projects will use fixed and snap frozen basal forebrain and temporal lobe tissue for immunohistochemistry, in situ hybridization, receptor binding and radioimmunoassay studies. The RADC will be the primary source of the moderately and severely impaired AD patients, and the RADC control study ad the Religious Order Study will be the source of the mildly impaired AD patients and control subjects. A thorough neuropathological evaluation will be performed on each case using standard diagnostic criteria for Alzheimer's disease (NIH consensus criteria), Parkinson's disease (CERAD), and related disorders. A direct entry data program will be used for all gross and microscopic neuropathological information. With the use of a specimen tracking software program (FreezerWorks), all tissue will be accurately indexed allowing rapid and reliable distribution of tissue to investigators. Close linkage will be maintained with the clinical core through monthly meetings to facilitate tissue distribution. The brain tissue and neuropathological data provided to investigators by the Neuropathology Core is critical for the success of the projects and for the provision of seminal information about nerve growth factor trophism, galanin remodeling, and altered tau conformation and the progression of Alzheimer's disease.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
3P01AG009466-09S1
Application #
6098281
Study Section
Project Start
1999-05-01
Project End
2000-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
9
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Rush University Medical Center
Department
Type
DUNS #
City
Chicago
State
IL
Country
United States
Zip Code
60612
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