The Animal Surgery core is designed to perform all the operative procedures required for the testing of each of the overall Group Hypotheses and for the Specific Aims of each of the four Projects. It is staffed by the Project director and the animal surgery technician. Objectives and functions of the core are as follows: 1) to perform all of the animal operative procedures needed for the entire Project; 2) to monitor the health status of the operated animals; 3) to schedule experiments and operations; 4) to assist in tissue removal and distribution to the Project laboratories; and 5) to prepare muscles that are used for the in vitro testing of contractile properties. Operations to be performed are standard, nerve-implant, and nerve-intact muscle grafts, nerve transections and permanent denervations, as well as injections of local anesthetics for the purpose of producing muscle fiber degeneration. The maintenance of the experimental animals, along with dealing with animal pathology, will be supervised by the unit for laboratory Animal Medicine at the University of Michigan.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG010821-09
Application #
6344581
Study Section
Project Start
2000-08-15
Project End
2001-06-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
9
Fiscal Year
2000
Total Cost
$154,432
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Kung, Theodore A; Cederna, Paul S; van der Meulen, Jack H et al. (2014) Motor unit changes seen with skeletal muscle sarcopenia in oldest old rats. J Gerontol A Biol Sci Med Sci 69:657-65
Dow, Douglas E; Cederna, Paul S; Hassett, Cheryl A et al. (2007) Electrical stimulation prior to delayed reinnervation does not enhance recovery in muscles of rats. Restor Neurol Neurosci 25:601-10
Dow, Douglas E; Carlson, Bruce M; Hassett, Cheryl A et al. (2006) Electrical stimulation of denervated muscles of rats maintains mass and force, but not recovery following grafting. Restor Neurol Neurosci 24:41-54
Dow, Douglas E; Dennis, Robert G; Faulkner, John A (2005) Electrical stimulation attenuates denervation and age-related atrophy in extensor digitorum longus muscles of old rats. J Gerontol A Biol Sci Med Sci 60:416-24
Borisov, Andrei B; Dedkov, Eduard I; Carlson, Bruce M (2005) Abortive myogenesis in denervated skeletal muscle: differentiative properties of satellite cells, their migration, and block of terminal differentiation. Anat Embryol (Berl) 209:269-79
Borisov, Andrei B; Dedkov, Eduard I; Carlson, Bruce M (2005) Differentiation of activated satellite cells in denervated muscle following single fusions in situ and in cell culture. Histochem Cell Biol 124:13-23
Dow, Douglas E; Faulkner, John A; Dennis, Robert G (2005) Distribution of rest periods between electrically generated contractions in denervated muscles of rats. Artif Organs 29:432-5
Dow, Douglas E; Cederna, Paul S; Hassett, Cheryl A et al. (2004) Number of contractions to maintain mass and force of a denervated rat muscle. Muscle Nerve 30:77-86
Pan, Jie; Ruest, Louis-Bruno; Xu, Suying et al. (2004) Immuno-characterization of the switch of peptide elongation factors eEF1A-1/EF-1alpha and eEF1A-2/S1 in the central nervous system during mouse development. Brain Res Dev Brain Res 149:1-8
Khalyfa, Abdelnaby; Carlson, Bruce M; Dedkov, Eduard I et al. (2003) Changes in protein levels of elongation factors, eEF1A-1 and eEF1A-2/S1, in long-term denervated rat muscle. Restor Neurol Neurosci 21:47-53

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