The projects will investigate five different aspects of the role of receptor ligand interactions in the regulation of lymphocyte function. Project 1 will determine the nature and significance of the intracellular and cell surface events that occur during the conjugate formation that follows when the T cell receptor recognizes the antigen peptide-class II MHC and will investigate the nature of the signals (lymphokines and interactions between cell bound molecules) conveyed to the B cell. The location and time of appearance of the relevant molecules and structures will be determined. This studies the cell biology of cell interactions. Project 2 will define the dynamics of T cell-B cell conjugate formation and dissociation and will determine the multiple parameters that regulate these events. The results obtained will be used to develop a model for the way in which various lymphocyte interactions occur in vivo. The approach here is to follow the activities of live cells by light microscopy. Project 3 will investigate the development and function of the subset of T cells bearing gamma/delta chain receptors for antigen in an attempt to understand the physiological significance of these cells. The project will examine the expression and function of T cell receptor genes in transfected cells and transgenic mice. Project 4 will investigate and characterize the various molecules carried in the membranes of activated T cells which are involved in transmitting signals to the B cell. The approach utilizes the techniques of membrane biochemistry to examine isolated components of the system. Project 5 will investigate the role of gangliosides in the modulation of signalling between T cells and B cells and seek to determine the physiological significance of the effects observed. The analysis here is biochemical and will compare the effects in model membranes and whole cell systems.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI023287-06
Application #
3091714
Study Section
Allergy & Clinical Immunology-1 (AITC)
Project Start
1986-05-01
Project End
1994-05-31
Budget Start
1991-06-15
Budget End
1992-05-31
Support Year
6
Fiscal Year
1991
Total Cost
Indirect Cost
Name
University of California San Diego
Department
Type
Schools of Medicine
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093
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