Abstract

The studies in this project will examine the roles of human papillomavirus gene expression in changes in the phenotype of epithelial cells infected with this virus. Organotypic cultures will be used as the in vitro method of choice since, in this tissue culture system, we can define the effect of expression of individual virus genes on the replication, stratification, keratinization and cornification of epithelial cells. Viral and cellular gene expression will be initially assessed by in situ hybridization methods and antigen detection on sections prepared from the organotypic cultures. Further studies will employ extraction of nucleic acids and proteins for examination by Southern and Northern blotting, polymerase chain reaction and radioimmune precipitation. Chemical and hormonal factors which may interact with HPV in the development of infection and progression of disease will also be incorporated in these studies. The factors involved in blocks to replication of HPVs which cannot currently be grown in vitro, will be examined.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI029363-02
Application #
3803951
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Fred Hutchinson Cancer Research Center
Department
Type
DUNS #
075524595
City
Seattle
State
WA
Country
United States
Zip Code
98109

  Publications

Watts, D H; Koutsky, L A; Holmes, K K et al. (1998) Low risk of perinatal transmission of human papillomavirus: results from a prospective cohort study. Am J Obstet Gynecol 178:365-73
Carter, J J; Koutsky, L A; Wipf, G C et al. (1996) The natural history of human papillomavirus type 16 capsid antibodies among a cohort of university women. J Infect Dis 174:927-36
Hagensee, M E; Carter, J J; Wipf, G C et al. (1995) Immunization of mice with HPV vaccinia virus recombinants generates serum IgG, IgM, and mucosal IgA antibodies. Virology 206:174-82
Ostrow, R S; Coughlin, S M; McGlennen, R C et al. (1995) Serological and molecular evidence of rhesus papillomavirus type 1 infections in tissues from geographically distinct institutions. J Gen Virol 76 ( Pt 2):293-9
Baken, L A; Koutsky, L A; Kuypers, J et al. (1995) Genital human papillomavirus infection among male and female sex partners: prevalence and type-specific concordance. J Infect Dis 171:429-32
Xi, L F; Demers, G W; Koutsky, L A et al. (1995) Analysis of human papillomavirus type 16 variants indicates establishment of persistent infection. J Infect Dis 172:747-55
Carter, J J; Hagensee, M B; Lee, S K et al. (1994) Use of HPV 1 capsids produced by recombinant vaccinia viruses in an ELISA to detect serum antibodies in people with foot warts. Virology 199:284-91
McDougall, J K (1994) Immortalization and transformation of human cells by human papillomavirus. Curr Top Microbiol Immunol 186:101-19
Xi, L F; Demers, W; Kiviat, N B et al. (1993) Sequence variation in the noncoding region of human papillomavirus type 16 detected by single-strand conformation polymorphism analysis. J Infect Dis 168:610-7
Merrick, D T; Gown, A M; Halbert, C L et al. (1993) Human papillomavirus-immortalized keratinocytes are resistant to the effects of retinoic acid on terminal differentiation. Cell Growth Differ 4:831-40

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