The Immunology Core will provide all immunological assays used in common among Projects 1 through 4. The Immunology Core will evaluate cell phenotypes and immune responses during vaccination and after virus challenge. These results, in conjunction with the evaluations made by the Virology Core, will be used by the projects to characterize the immune responses to the different vaccines and to identify correlates of protective immunity. There are three specific aims.
Aim 1. To serve as a receiving point for rhesus macaque blood specimens drawn by the IHV division of Animal Models, coding, cataloguing and processing these specimens for distribution to the Immunology and Virology Cores or archiving processed specimens, as appropriate, for eventual distribution. Specimens processing will including isolating PBMCs and plasma from heparinized or citrate-treated peripheral blood flow by Ficoll-Hypaque density gradient centrifugation.
Aim 2. To perform studies of rhesus macaque cell phenotype and lymphocyte function. Studies will include: a. flow cytometry evaluations of the surface phenotypic markers CD3 (pan T cell), CD4 (T cell subset), CD8 (T cell subset), CD20 (B cells), and CD14 (monocytes), b. determinations of polyclonal and antigen specific proliferative responses, c. preparing and harvesting culture supernatants from T cells activated by polyclonal or antigen-specific stimuli for chemokine production by ELISA, d. preparing and harvesting culture supernatants from T cells activated by polyclonal or antigen-specific stimuli for virus suppressor factors (VSFs), e. HIV or SIV-specific cytotoxic T lymphocyte (CTL) assays f. to evaluate the presence of virus- specific serum antibodies by ELISA or Western blot analysis, Aim 3. To collate data from assays initiated by the Immunology Core and Transmit it to the Project Leaders and the Administrative Core.
|Bagley, Kenneth C; Abdelwahab, Sayed F; Tuskan, Robert G et al. (2006) Cholera toxin indirectly activates human monocyte-derived dendritic cells in vitro through the production of soluble factors, including prostaglandin E(2) and nitric oxide. Clin Vaccine Immunol 13:106-15|
|Bagley, Kenneth C; Abdelwahab, Sayed F; Tuskan, Robert G et al. (2005) Pasteurella multocida toxin activates human monocyte-derived and murine bone marrow-derived dendritic cells in vitro but suppresses antibody production in vivo. Infect Immun 73:413-21|
|Bagley, Kenneth C; Abdelwahab, Sayed F; Tuskan, Robert G et al. (2004) Calcium signaling through phospholipase C activates dendritic cells to mature and is necessary for the activation and maturation of dendritic cells induced by diverse agonists. Clin Diagn Lab Immunol 11:77-82|
|Abdelwahab, Sayed F; Cocchi, Fiorenza; Bagley, Kenneth C et al. (2003) HIV-1-suppressive factors are secreted by CD4+ T cells during primary immune responses. Proc Natl Acad Sci U S A 100:15006-10|
|Bagley, K C; Shata, M T; Onyabe, D Y et al. (2003) Immunogenicity of DNA vaccines that direct the coincident expression of the 120 kDa glycoprotein of human immunodeficiency virus and the catalytic domain of cholera toxin. Vaccine 21:3335-41|
|Bagley, Kenneth C; Abdelwahab, Sayed F; Tuskan, Robert G et al. (2003) An enzymatically active a domain is required for cholera-like enterotoxins to induce a long-lived blockade on the induction of oral tolerance: new method for screening mucosal adjuvants. Infect Immun 71:6850-6|
|Bagley, Kenneth C; Abdelwahab, Sayed F; Tuskan, Robert G et al. (2002) Pertussis toxin and the adenylate cyclase toxin from Bordetella pertussis activate human monocyte-derived dendritic cells and dominantly inhibit cytokine production through a cAMP-dependent pathway. J Leukoc Biol 72:962-9|
|Bagley, Kenneth C; Abdelwahab, Sayed F; Tuskan, Robert G et al. (2002) Cholera toxin and heat-labile enterotoxin activate human monocyte-derived dendritic cells and dominantly inhibit cytokine production through a cyclic AMP-dependent pathway. Infect Immun 70:5533-9|
|Devico, Anthony L; Fouts, Timothy R; Shata, Mohamed T et al. (2002) Development of an oral prime-boost strategy to elicit broadly neutralizing antibodies against HIV-1. Vaccine 20:1968-74|
|Kamin-Lewis, R; Abdelwahab, S F; Trang, C et al. (2001) Perforin-low memory CD8+ cells are the predominant T cells in normal humans that synthesize the beta -chemokine macrophage inflammatory protein-1beta. Proc Natl Acad Sci U S A 98:9283-8|