Abstract

The Histology and Immunohistochemistry Core will evaluate tissues from mutant and wild type mice for projects 2, 3 and 4. The purpose will be to assess any histochemical or cellular abnormalities at the light micrographic level. Bone, cartilage and other tissues will be surveyed by routine histological techniques, as well as by immunofluorescent approaches for matrix molecules including the collagen types, osteopontin, osteocalcin and alkaline phosphatase. Comparisons will be made between normal tissues and those eliciting gross or microanatomical variations. Since the targeted genes focus upon bone and cartilage macromolecules, the histological evaluations will also focus upon bone, cartilage and surrounding tissues. Nevertheless, routine surveys of other tissues will also be made. The core will also provide appropriate micrographs and slides for publication and presentation. Since evaluation of defects brought about by genetic manipulations described in this proposal at the microanatomical level are vital, the Histology and Immunohistochemistry Core is an essential part of this program.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Program Projects (P01)
Project #
1P01AR042919-01A1
Application #
5206358
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Coustry, Francoise; Oh, Chun-do; Hattori, Takako et al. (2010) The dimerization domain of SOX9 is required for transcription activation of a chondrocyte-specific chromatin DNA template. Nucleic Acids Res 38:6018-28
Nuka, S; Zhou, W; Henry, S P et al. (2010) Phenotypic characterization of epiphycan-deficient and epiphycan/biglycan double-deficient mice. Osteoarthritis Cartilage 18:88-96
Hattori, Takako; Coustry, Francoise; Stephens, Shelley et al. (2008) Transcriptional regulation of chondrogenesis by coactivator Tip60 via chromatin association with Sox9 and Sox5. Nucleic Acids Res 36:3011-24
Kimura, Hiroaki; Akiyama, Haruhiko; Nakamura, Takashi et al. (2007) Tenascin-W inhibits proliferation and differentiation of preosteoblasts during endochondral bone formation. Biochem Biophys Res Commun 356:935-41
Akiyama, Haruhiko; Stadler, H Scott; Martin, James F et al. (2007) Misexpression of Sox9 in mouse limb bud mesenchyme induces polydactyly and rescues hypodactyly mice. Matrix Biol 26:224-33
Lee, Hu-Hui; Behringer, Richard R (2007) Conditional expression of Wnt4 during chondrogenesis leads to dwarfism in mice. PLoS One 2:e450
Govoni, Kristen E; Lee, Seong Keun; Chung, Yoon-Sok et al. (2007) Disruption of insulin-like growth factor-I expression in type IIalphaI collagen-expressing cells reduces bone length and width in mice. Physiol Genomics 30:354-62
Gebhard, Sonja; Hattori, Takako; Bauer, Eva et al. (2007) BAC constructs in transgenic reporter mouse lines control efficient and specific LacZ expression in hypertrophic chondrocytes under the complete Col10a1 promoter. Histochem Cell Biol 127:183-94
Ovchinnikov, Dmitry A; Selever, Jennifer; Wang, Ying et al. (2006) BMP receptor type IA in limb bud mesenchyme regulates distal outgrowth and patterning. Dev Biol 295:103-15
Steiglitz, Barry M; Kreider, Jaclynn M; Frankenburg, Elizabeth P et al. (2006) Procollagen C proteinase enhancer 1 genes are important determinants of the mechanical properties and geometry of bone and the ultrastructure of connective tissues. Mol Cell Biol 26:238-49

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