Abstract

We have pursued our analysis of the anti-inflammatory properties of heparin, depleted of anticoagulant activity and administered orally or intravenously on delayed-type hypersensitivity reactions, and demonstrated its interference with the migration of inflammatory cells into the challenged site. We have documented the influence that immunoglobulin-bearing B cells have on the development of the suppressor T-cell repertoire by demonstrating the role of Ig-bearing B cells on the development of T-cell idiotypes. A requirement for an intracellular processing step by accessory cells previous to antigen presentation to T cells was demonstrated for a broad group of native antigens, among which were the determinants of Listeria monocytogenes and hen egg liposome. The suppression by T cells of immunoglobulin synthesis by mouse myeloma was subjected to further study. The molecular parameters essential for the initiation of secondary in vitro allogeneic cytolytic T-cell response to Class I MHC antigens were investigated using purified antigens and liposomes. The density of H-2 proteins was found to be essential for effective recognition. (MB)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA014723-15
Application #
3092650
Study Section
Cancer Special Program Advisory Committee (CAK)
Project Start
1978-06-01
Project End
1988-05-31
Budget Start
1987-06-01
Budget End
1988-05-31
Support Year
15
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Harvard University
Department
Type
Schools of Medicine
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Wasik, M A; Beller, D I (1989) Induction of macrophage membrane interleukin 1 expression by T-cell dependent and T-cell independent pathways is inhibited by cyclosporin A. Clin Immunol Immunopathol 52:331-40
Wright Jr, T C; Castellot Jr, J J; Petitou, M et al. (1989) Structural determinants of heparin's growth inhibitory activity. Interdependence of oligosaccharide size and charge. J Biol Chem 264:1534-42
Karnovsky, M J; Wright Jr, T C; Castellot Jr, J J et al. (1989) Heparin, heparan sulfate, smooth muscle cells, and atherosclerosis. Ann N Y Acad Sci 556:268-81
Kurt-Jones, E A; Liano, D; HayGlass, K A et al. (1988) The role of antigen-presenting B cells in T cell priming in vivo. Studies of B cell-deficient mice. J Immunol 140:3773-8
Williams, W W; Falo Jr, L D; Lu, C Y et al. (1988) Effects of in vivo monoclonal anti-I-A antibody treatment in neonatal mice on intrathymic and peripheral class II antigen expression. Cell Immunol 111:126-38
Reiser, H; Yeh, E T; Coligan, J et al. (1988) Structure and function of the TAP protein and related Ly-6-linked molecules. Year Immunol 3:80-8
Sy, M S; Benacerraf, B (1988) Suppressor T cells, immunoglobulin and Igh restriction. Immunol Rev 101:133-48
Yeh, E T; Reiser, H; Bamezai, A et al. (1988) TAP transcription and phosphatidylinositol linkage mutants are defective in activation through the T cell receptor. Cell 52:665-74
Bamezai, A; Reiser, H; Rock, K L (1988) T cell receptor/CD3 negative variants are unresponsive to stimulation through the Ly-6 encoded molecule, TAP. J Immunol 141:1423-8
Wasik, M A; Donnelly, R P; Beller, D I (1988) Lymphokine-independent induction of macrophage membrane IL-1 by autoreactive T cells recognizing either class I or class II MHC determinants. J Immunol 141:3456-62

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