Abstract

This core will provide centralized services and reagents to the research projects in the Program Project and will also provide shared instrumentation. As established in the prior proposal, the core will continue to provide large scale preparation of cultured cells, production of EGF receptor-rich vesicles from A-431 cells, preparation of mouse-derived EGF, preparation of 125I-EGF and 125I-protein A, and preparation of antisera. In addition, the core will expand its services to act as a repository and source of various vectors and the appropriate cell lines necessary for protein expression in either eukaryotic or prokaryotic systems. The core will provide technical support for initial protein expression studies and for large scale protein production and purification. In terms of instrumentation, the core will provide access to fermentation and centrifugation equipment for large scale protein expression, to a Phast Electrophoresis system for rapid protein detection, to a HPLC system for rapid, large scale protein purification, and the necessary technical assistance.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
2P01CA043720-06
Application #
3795451
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212

  Publications

Liao, J; Piwien-Pilipuk, G; Ross, S E et al. (1999) CCAAT/enhancer-binding protein beta (C/EBPbeta) and C/EBPdelta contribute to growth hormone-regulated transcription of c-fos. J Biol Chem 274:31597-604
Kamat, A; Carpenter, G (1997) Phospholipase C-gamma1: regulation of enzyme function and role in growth factor-dependent signal transduction. Cytokine Growth Factor Rev 8:109-17
Penta, K; Carpenter, G (1997) Interaction of phospholipase C-gamma with activated growth factor receptor tyrosine kinases. Adv Exp Med Biol 400B:971-81
Ji, Q S; Winnier, G E; Niswender, K D et al. (1997) Essential role of the tyrosine kinase substrate phospholipase C-gamma1 in mammalian growth and development. Proc Natl Acad Sci U S A 94:2999-3003
Coats, S R; Pledger, W J; Awazu, M et al. (1996) Detergent solubility defines an alternative itinerary for a subpopulation of PDGF beta receptors. J Cell Physiol 168:412-23
Horstman, D A; DeStefano, K; Carpenter, G (1996) Enhanced phospholipase C-gamma1 activity produced by association of independently expressed X and Y domain polypeptides. Proc Natl Acad Sci U S A 93:7518-21
Scoggins, R M; Summerfield, A E; Stein, R A et al. (1996) 5'-(p-fluorosulfonylbenzoyl)-2'(or 3')-(methylanthraniloyl)adenosine, fluorescent affinity labels for adenine nucleotide binding sites: interaction with the kinase active site of the receptor for epidermal growth factor. Biochemistry 35:9197-203
Winston, J T; Coats, S R; Wang, Y Z et al. (1996) Regulation of the cell cycle machinery by oncogenic ras. Oncogene 12:127-34
Tong, K; Guyer, C A; Staros, J V (1996) Steric constraints in the recognition of peptide substrates for the epidermal growth factor receptor kinase. Int J Pept Protein Res 47:219-26
Stein, R A; Staros, J V (1996) Thermal inactivation of the protein tyrosine kinase of the epidermal growth factor receptor. Biochemistry 35:2878-84

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