Core B core Director Colcher, David Principal investigator: Raubitschek, AndrewDESCRIPTION:The principal objective of the Radiopharmacy and Assays Core (Core B) is to support Project 1:Radioimmunotherapy: Clinical Studies, Project 2: Pretargeting with anti-CEA Antibodies for Therapy, andProject 3: CEA Targeted Immunotherapeutics by providing expertise and facilities to prepare radiolabeledantibodies and quality control for therapeutic and imaging reagents. Yttrium-90 radiolabeledchimeric/humanized T84.66 anti-CEA antibody will be used in the clinical trials for colon and lung cancersatients, and yttrium-90 radiolabeled Herceptin will be used in breast cancer patients at the City of HopeNational Medical Center, lndium-111 radiolabeled chimeric/humanized T84.66 antibody, Avastin andHerceptin will be used for imaging and dosimetry studies. The radiopharmacy will support Project 1 byproviding the radiolabeled antibodies and will provide quality control for these reagents.This Core is also responsible for the analysis of patient (and animal) blood samples to evaluate the bloodclearance and stability of the administered products. Patient sera samples are also analyzed to monitor thepatient's immune response to the injected antibody constructs.Core B also performs the radiolabeling of all the antibodies, engineered antibodies, chemically modifiedantibodies and haptens for preclinical in vitro and in vivo use. Procedures involved in preparation of theradiopharmaceutical agents include chelate conjugate chemistry and conjugation as well as the antibodyradiolabeling. As part of its core activity the Radiopharmacy also develops the monoclonal antibodyconstruct conjugation methodologies for the clinical grade materials to be prepared in the cGMP facility forthe Phase I trials.Core B will be associatedwith all three Projects as well as Cores A, C, D and E in this application.
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