Core - Protein Expression Facility

Ware, Carl F.

Abstract

The Protein Expression Facility will provide investigators a common source of highly purified proteins and the technical knowledge for use of these proteins to meet the experimental objectives of this Program Project. The Protein Expression Facility will consolidate the efforts of five laboratories into one facility, eliminating unnecessary duplication of technical expertise and decreasing expenses for technical staff, while providing quality control for critical reagents. The Facility will provide functional fragments of TNF receptor and related receptors and their signaling molecules using baculovirus and bacterial expression systems. A formalized procedure for establishing priority reagent requests among the investigators is described. The Facility will be located at UC Riverside.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Cancer Institute (NCI)
• Type: Research Program Projects (P01)
• Project #: 1P01CA069381-01A1
• Application #: 5209522
• Support Year: 1
• Fiscal Year: 1996

Publications

Xu, X-P; Zhai, D; Kim, E et al. (2013) Three-dimensional structure of Bax-mediated pores in membrane bilayers. Cell Death Dis 4:e683

Chipuk, Jerry E; McStay, Gavin P; Bharti, Archana et al. (2012) Sphingolipid metabolism cooperates with BAK and BAX to promote the mitochondrial pathway of apoptosis. Cell 148:988-1000

Fujikura, D; Ito, M; Chiba, S et al. (2012) CLIPR-59 regulates TNF-?-induced apoptosis by controlling ubiquitination of RIP1. Cell Death Dis 3:e264

Zervoudi, Efthalia; Papakyriakou, Athanasios; Georgiadou, Dimitra et al. (2011) Probing the S1 specificity pocket of the aminopeptidases that generate antigenic peptides. Biochem J 435:411-20

Pop, Cristina; Oberst, Andrew; Drag, Marcin et al. (2011) FLIP(L) induces caspase 8 activity in the absence of interdomain caspase 8 cleavage and alters substrate specificity. Biochem J 433:447-457

Cheung, Timothy C; Ware, Carl F (2011) The canonical and unconventional ligands of the herpesvirus entry mediator. Adv Exp Med Biol 691:353-62

Garrison, Jason B; Correa, Ricardo G; Gerlic, Motti et al. (2011) ARTS and Siah collaborate in a pathway for XIAP degradation. Mol Cell 41:107-16

Lu, Jennifer V; Weist, Brian M; van Raam, Bram J et al. (2011) Complementary roles of Fas-associated death domain (FADD) and receptor interacting protein kinase-3 (RIPK3) in T-cell homeostasis and antiviral immunity. Proc Natl Acad Sci U S A 108:15312-7

Ponder, Elizabeth L; Albrow, Victoria E; Leader, Brittany A et al. (2011) Functional characterization of a SUMO deconjugating protease of Plasmodium falciparum using newly identified small molecule inhibitors. Chem Biol 18:711-21

Timmer, John C; Salvesen, Guy S (2011) N-terminomics: a high-content screen for protease substrates and their cleavage sites. Methods Mol Biol 753:243-55