The purpose of the high-throughput screening (HTS) core laboratory (Core B) is to create and conduct HTSscreens of small molecules to identify those that can become leads for developing cancer therapeutics ortools in cancer research. In addition, the HTS laboratory conducts genomic RNAi screens to identify noveltargets for developing cancer therapeutics.Core B manages the UTSW chemical compound file currently including more than 200,000 small drug-likeorganic compounds. Core B develops assays for the projects of the PPG,screens them and analyzesscreening data. The HTS lab conducts secondary screens required to validate compounds of interestidentified during primary screening experiments. Working with the investigators of the PPG, scientists inCore B and the Chemistry Core prioritize the compounds of interest identified in HTS experiments andmanage resupply and storage of those compounds. The HTS core maintains an Oracle database ofscreening data so that the activity of compounds of interest in any HTS experiment can be compared to allother HTS experiments conducted with the UTSW compound file. The HTS Core also manages andconducts HTS screening experiments of a human genomic siRNA library for the Projects of the PPG.In addition to conducting HTS screens for members of this NCI funded PPG, Core B trains and advisesmembers of the Comprehensive Cancer Center at UT Southwestern on the development and execution ofsmall molecule and genomic siRNA HTS experiments and stores the data from such experiments as part ofthe UTSW screening database.The HTS facility advances cancer research by providing cancer researchers access to hundreds ofthousands of structurally diverse compounds from which leads for drug therapeutics or tools for cancerresearch can be identified.
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