Core Component B, the Signaling and Motility Assays Core, will provide the expertise, protocols, analysis and interpretation of in vitro assays that examine the individual behavior, and interaction of tumor and stromal cells. The Core performs assays examining two-dimensional motile cell behavior, three-dimensional invasion, angiogenesis and transendothelial migration. These assays permit a comprehensive examination of cell behavior during chemotaxis, and provide a rapid and complete analysis of the invasive capabilities of a given cell type prior to costly and time-consuming animal studies. Moreover these in vitro assays support and complement the in vivo studies provided by the Intravital Imaging, Invasive Cell Collection and Expression Profiling Core (Core A). Core B will also act as a liaison for the use of unique protein biosensors and for assays examining invadopod formation so that the application of these technologies is uniform amongst the projects. The in vitro assays supported by Core B provide much needed information about cellular behavior and mechanism during tumor cell invasion and intravasation.The leading cause of death in cancer patients is the ability of tumor cells to leave the primary tumor, travel to distant sites within the body and form secondary tumors. The assays provided by this Core specifically examine the individual behavior and interaction of tumor and stromal cells, and will provide the foundation for understanding the mechanisms regulating tumor cell motility and invasion.
| Donnelly, Sara K; Miskolci, Veronika; Garrastegui, Alice M et al. (2018) Characterization of Genetically Encoded FRET Biosensors for Rho-Family GTPases. Methods Mol Biol 1821:87-106 |
| Entenberg, David; Voiculescu, Sonia; Guo, Peng et al. (2018) A permanent window for the murine lung enables high-resolution imaging of cancer metastasis. Nat Methods 15:73-80 |
| Norwood Toro, Laura E; Wang, Yarong; Condeelis, John S et al. (2018) Myosin-IIA heavy chain phosphorylation on S1943 regulates tumor metastasis. Exp Cell Res 370:273-282 |
| Bresnick, Anne R (2018) S100 proteins as therapeutic targets. Biophys Rev 10:1617-1629 |
| Suyama, Kimita; Yao, Jiahong; Liang, Huizhi et al. (2018) An Akt3 Splice Variant Lacking the Serine 472 Phosphorylation Site Promotes Apoptosis and Suppresses Mammary Tumorigenesis. Cancer Res 78:103-114 |
| Pastoriza, Jessica M; Karagiannis, George S; Lin, Juan et al. (2018) Black race and distant recurrence after neoadjuvant or adjuvant chemotherapy in breast cancer. Clin Exp Metastasis 35:613-623 |
| Arwert, Esther N; Harney, Allison S; Entenberg, David et al. (2018) A Unidirectional Transition from Migratory to Perivascular Macrophage Is Required for Tumor Cell Intravasation. Cell Rep 23:1239-1248 |
| Gizzi, Anthony S; Grove, Tyler L; Arnold, Jamie J et al. (2018) A naturally occurring antiviral ribonucleotide encoded by the human genome. Nature 558:610-614 |
| Karagiannis, George S; Condeelis, John S; Oktay, Maja H (2018) Chemotherapy-induced metastasis: mechanisms and translational opportunities. Clin Exp Metastasis 35:269-284 |
| Yang, Ming; McKay, Daniel; Pollard, Jeffrey W et al. (2018) Diverse Functions of Macrophages in Different Tumor Microenvironments. Cancer Res 78:5492-5503 |
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