Abstract

It has been postulated by various investigators that cells which possess enzyme terminal deoxynucleotidyl transferase in the bone marrow of young adult experimental mammals and birds are T cell progenitors. Recently, an in vitro culture method which promotes the growth of rat bone marrow TdT-positive T cell progenitor cells has been established. This culture system requires a continuous presence of an adherent feeder layer from mouse bone marrow cells. TdT-positive cells from rat bone marrow selectively proliferate in the suspension phase of the culture media when a healthy mouse bone marrow feeder layer is present. We have also established a culture method for growing thymic epithelial cells in serum-free culture conditions. From these primary culture, a cell line (TEA3A1 cells) was established. TEA3A1 cells produce biological activity which stimulates the (3H)-TdR incorporation of ConA-stimulated thymocytes. This activity is different from I1-2. In this application we propose to examine the developmental potential of bone marrow TdT-positive cells and the functional capacity of various thymic stromal cells including TEA3A1 cells to support the growth and/or differentiation of TdT-positive cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK038639-03
Application #
3876115
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
Adirondack Biomedical Research Institute
Department
Type
DUNS #
City
Lake Placid
State
NY
Country
United States
Zip Code
12946

  Publications

Yu, Y; Sato, J D (1999) MAP kinases, phosphatidylinositol 3-kinase, and p70 S6 kinase mediate the mitogenic response of human endothelial cells to vascular endothelial growth factor. J Cell Physiol 178:235-46
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Liu, P; Lalor, D; Bowser, S S et al. (1998) Regulation of arachidonic acid release and prostaglandin E2 production in thymic epithelial cells by ATPgammaS and transforming growth factor-alpha. Cell Immunol 188:81-8
Ye, H; Serrero, G (1998) Stimulation of adipose differentiation related protein (ADRP) expression by ibuprofen and indomethacin in adipocyte precursors and in adipocytes. Biochem J 330 ( Pt 2):803-9
Serrero, G; Lepak, N M (1997) Prostaglandin F2alpha receptor (FP receptor) agonists are potent adipose differentiation inhibitors for primary culture of adipocyte precursors in defined medium. Biochem Biophys Res Commun 233:200-2
Kapron, J T; Hilliard, G M; Lakins, J N et al. (1997) Identification and characterization of glycosylation sites in human serum clusterin. Protein Sci 6:2120-33
Myoken, Y; Myoken, Y; Okamoto, T et al. (1997) Immunohistochemical localization of fibroblast growth factor-1 (FGF-1), FGF-2 and fibroblast growth factor receptor-1 (FGFR-1) in pleomorphic adenoma of the salivary glands. J Oral Pathol Med 26:17-22
McKeehan, K; McKeehan, W I; Xu, J et al. (1996) Kinase-inactive splice variants of the activin type I receptor. In Vitro Cell Dev Biol Anim 32:131-4
Myoken, Y; Myoken, Y; Okamoto, T et al. (1996) Expression of fibroblast growth factor-1 (FGF-1), FGF-2 and FGF receptor-1 in a human salivary-gland adenocarcinoma cell line: evidence of growth. Int J Cancer 65:650-7
Serrero, G; Lepak, N (1996) Endocrine and paracrine negative regulators of adipose differentiation. Int J Obes Relat Metab Disord 20 Suppl 3:S58-64

Showing the most recent 10 out of 65 publications