Human hepatoma cells secrete low molecular weight, acid-stable protease inhibitors which have stimulatory effects on endothelial cell growth and may inhibit the immune response to tumors. Recombinant cDNA clones for human pancreatic secretory trypsin inhibitor (HPTI) and urinary glycoprotein inhibitor (HI-30) will be isolated and characterized from a human hepatoma cDNA library. The complexity and differences between mRNA species coding for HPTI and HI-30 in normal hepatocytes and hepatoma cells will be determined. HI-30-related antigens in the medium of liver cells are complex as determined by immunochemical analysis. If HI-30 antigens arise by post-translational modification of a single gene product, the complete amino acid sequence of the product will be deduced from cDNA sequence. If HI-30 antigens are separate gene products, unique cDNA probes will be constructed for unique products. cDNA probes will be used to quantitate expression of mRNAs for HPTI and HI-30 antigens in normal hepatocytes and hepatoma cells. The effect of heparin-binding growth factors (HBGF) on expression of HPTI and HI-30 antigens and mRNA will be compared to other hormonal effectors of hepatocyte secretory proteins.

Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
1992
Total Cost
Indirect Cost
Name
Adirondack Biomedical Research Institute
Department
Type
DUNS #
City
Lake Placid
State
NY
Country
United States
Zip Code
12946
Yu, Y; Sato, J D (1999) MAP kinases, phosphatidylinositol 3-kinase, and p70 S6 kinase mediate the mitogenic response of human endothelial cells to vascular endothelial growth factor. J Cell Physiol 178:235-46
Herley, M T; Yu, Y; Whitney, R G et al. (1999) Characterization of the VEGF binding site on the Flt-1 receptor. Biochem Biophys Res Commun 262:731-8
Liu, P; Lalor, D; Bowser, S S et al. (1998) Regulation of arachidonic acid release and prostaglandin E2 production in thymic epithelial cells by ATPgammaS and transforming growth factor-alpha. Cell Immunol 188:81-8
Ye, H; Serrero, G (1998) Stimulation of adipose differentiation related protein (ADRP) expression by ibuprofen and indomethacin in adipocyte precursors and in adipocytes. Biochem J 330 ( Pt 2):803-9
Serrero, G; Lepak, N M (1997) Prostaglandin F2alpha receptor (FP receptor) agonists are potent adipose differentiation inhibitors for primary culture of adipocyte precursors in defined medium. Biochem Biophys Res Commun 233:200-2
Kapron, J T; Hilliard, G M; Lakins, J N et al. (1997) Identification and characterization of glycosylation sites in human serum clusterin. Protein Sci 6:2120-33
Myoken, Y; Myoken, Y; Okamoto, T et al. (1997) Immunohistochemical localization of fibroblast growth factor-1 (FGF-1), FGF-2 and fibroblast growth factor receptor-1 (FGFR-1) in pleomorphic adenoma of the salivary glands. J Oral Pathol Med 26:17-22
Myoken, Y; Myoken, Y; Okamoto, T et al. (1996) Expression of fibroblast growth factor-1 (FGF-1), FGF-2 and FGF receptor-1 in a human salivary-gland adenocarcinoma cell line: evidence of growth. Int J Cancer 65:650-7
Serrero, G; Lepak, N (1996) Endocrine and paracrine negative regulators of adipose differentiation. Int J Obes Relat Metab Disord 20 Suppl 3:S58-64
Matsuzaki, K; Kan, M; McKeehan, W L (1996) Reconstitution of a pentameric complex of dimeric transforming growth factor beta ligand and a type I, II, III receptor in baculoviral-infected insect cells. In Vitro Cell Dev Biol Anim 32:345-60

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