Transgenic Mice Core

Chalkley, Roger

Abstract

This core will provide a centralized service for producing transgenic mice. Specifically, the core will undertake the following procedures: (1) microinjection of fertilized one-cell mouse eggs with specific DNA constructs prepared by Program Project investigators, (2) return the eggs to pseudopregnant foster mothers, (3) screen for transgenic offspring by Southern analysis of DNA isolated from tails, and (4) assist in the dissection and analysis of tissues from transgenic mice and embryos. Therefore, in addition to providing an increasingly important research tool for members of the DRTC who may wish to establish the technique, or collaborate on projects related to the expression and mode of action of factors regulating growth and differentiation in vivo.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
• Type: Research Program Projects (P01)
• Project #: 1P01DK042502-01
• Application #: 3876338
• Support Year: 1
• Fiscal Year: 1990

Institution

• Name: Vanderbilt University Medical Center
• DUNS #: 004413456
• City: Nashville
• State: TN
• Country: United States
• Zip Code: 37212

Publications

Zhang, Juliet; Weinrich, Jarret A P; Russ, Jeffrey B et al. (2017) A Role for Dystonia-Associated Genes in Spinal GABAergic Interneuron Circuitry. Cell Rep 21:666-678

Krah, Nathan M; De La O, Jean-Paul; Swift, Galvin H et al. (2015) The acinar differentiation determinant PTF1A inhibits initiation of pancreatic ductal adenocarcinoma. Elife 4:

Baeyens, Luc; Lemper, Marie; Leuckx, Gunter et al. (2014) Transient cytokine treatment induces acinar cell reprogramming and regenerates functional beta cell mass in diabetic mice. Nat Biotechnol 32:76-83

Liu, Jing; Willet, Spencer G; Bankaitis, Eric D et al. (2013) Non-parallel recombination limits Cre-LoxP-based reporters as precise indicators of conditional genetic manipulation. Genesis 51:436-42

Kopinke, Daniel; Brailsford, Marisa; Pan, Fong Cheng et al. (2012) Ongoing Notch signaling maintains phenotypic fidelity in the adult exocrine pancreas. Dev Biol 362:57-64

Gu, Yanyun; Lindner, Jill; Kumar, Anil et al. (2011) Rictor/mTORC2 is essential for maintaining a balance between beta-cell proliferation and cell size. Diabetes 60:827-37

Pound, Lynley D; Hang, Yan; Sarkar, Suparna A et al. (2011) The pancreatic islet ?-cell-enriched transcription factor Pdx-1 regulates Slc30a8 gene transcription through an intronic enhancer. Biochem J 433:95-105

Papizan, James B; Singer, Ruth A; Tschen, Shuen-Ing et al. (2011) Nkx2.2 repressor complex regulates islet ?-cell specification and prevents ?-to-?-cell reprogramming. Genes Dev 25:2291-305

Artner, Isabella; Hang, Yan; Mazur, Magdalena et al. (2010) MafA and MafB regulate genes critical to beta-cells in a unique temporal manner. Diabetes 59:2530-9

Masui, Toshihiko; Swift, Galvin H; Deering, Tye et al. (2010) Replacement of Rbpj with Rbpjl in the PTF1 complex controls the final maturation of pancreatic acinar cells. Gastroenterology 139:270-80