Abstract

The objective of this Core Unit is to provide the investigators of the program project with a faculty allowing biological testing of the function of cloned hematopoietic stem cell genes. The facility is located in a microinjection suite on the -2 floor of the K wing of the UW Health Sciences Center of the University of Washington. It is fully equipped and has two microinjection units. A tissue culture room is located adjacent to this facility. The mouse rooms are located in the same area. The core unit has extensive experience in producing transgenic mice using plasmid or cosmid-sized constructs as well as with yeast artificial chromosomes (YACs). The personnel have experience with production of chimeric knockout mice. Core D will initially produce transgenic and knockout mice for Projects 1 and 2 and it will also be extensively utilized by Projects 3 and 4 once the genes responsible for familial leukemia or cyclic hematopoiesis have been cloned.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK055820-02
Application #
6450354
Study Section
Special Emphasis Panel (ZDK1)
Project Start
2001-05-01
Project End
2002-04-30
Budget Start
Budget End
Support Year
2
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Type
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Xu, Huilei; Schaniel, Christoph; Lemischka, Ihor R et al. (2010) Toward a complete in silico, multi-layered embryonic stem cell regulatory network. Wiley Interdiscip Rev Syst Biol Med 2:708-33
Macarthur, Ben D; Ma'ayan, Avi; Lemischka, Ihor R (2009) Systems biology of stem cell fate and cellular reprogramming. Nat Rev Mol Cell Biol 10:672-81
Chang, Kai-Hsin; Nelson, Angelique M; Cao, Hua et al. (2006) Definitive-like erythroid cells derived from human embryonic stem cells coexpress high levels of embryonic and fetal globins with little or no adult globin. Blood 108:1515-23
Duan, Zhijun; Horwitz, Marshall (2005) Gfi-1 takes center stage in hematopoietic stem cells. Trends Mol Med 11:49-52
Richard, Robert E; Siritanaratkul, Noppadol; Jonlin, Erica et al. (2005) Collection of blood stem cells from patients with sickle cell anemia. Blood Cells Mol Dis 35:384-8
Li, Feng-Qian; Person, Richard E; Takemaru, Ken-Ichi et al. (2004) Lymphoid enhancer factor-1 links two hereditary leukemia syndromes through core-binding factor alpha regulation of ELA2. J Biol Chem 279:2873-84
Duan, Zhijun; Li, Feng-Qian; Wechsler, Jeremy et al. (2004) A novel notch protein, N2N, targeted by neutrophil elastase and implicated in hereditary neutropenia. Mol Cell Biol 24:58-70
Doan, Loretta L; Porter, Susan D; Duan, Zhijun et al. (2004) Targeted transcriptional repression of Gfi1 by GFI1 and GFI1B in lymphoid cells. Nucleic Acids Res 32:2508-19
Escher, Robert; Jones, Antonia; Hagos, Fitsum et al. (2004) Chromosome band 16q22-linked familial AML: exclusion of candidate genes, and possible disease risk modification by NQO1 polymorphisms. Genes Chromosomes Cancer 41:278-82
Zhao, Shengming; Weinreich, Michael A; Ihara, Kenji et al. (2004) In vivo selection of genetically modified erythroid cells using a jak2-based cell growth switch. Mol Ther 10:456-68

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