Abstract

The purpose of the proposed Imaging Core is to provide Program investigators access to state-of-the-art multicolor confocal and two- photon excitation microscopy, and associated digital imaging processing and 3-D reconstruction software in the laboratory of Dr. David Piston. The capabilities of this instrumentation and software greatly exceed those currently available to all Vanderbilt faculty on a fee-for-service basis in the Vanderbilt Cell Imaging Resource Core. These enhanced imaging capabilities are required for individual projects that comprise the program. Dr. Piston is an internationally recognized expert in digital might microscopy and quantitative imaging. By engaging him as a full scientific participant in the Program, collaborations and interactions between Dr. Piston and the project leaders will undoubtedly result in the development of new methods and approaches for studying C. elegans.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK058212-03
Application #
6618906
Study Section
Special Emphasis Panel (ZDK1)
Project Start
2002-09-01
Project End
2003-08-31
Budget Start
Budget End
Support Year
3
Fiscal Year
2002
Total Cost
$159,855
Indirect Cost

  Institution

Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212

  Publications

Benninger, Richard K P; Piston, David W (2014) Cellular communication and heterogeneity in pancreatic islet insulin secretion dynamics. Trends Endocrinol Metab 25:399-406
Kumar, Ankur N; Short, Kurt W; Piston, David W (2013) A motion correction framework for time series sequences in microscopy images. Microsc Microanal 19:433-50
Ustione, Alessandro; Piston, David W (2012) Dopamine synthesis and D3 receptor activation in pancreatic ?-cells regulates insulin secretion and intracellular [Ca(2+)] oscillations. Mol Endocrinol 26:1928-40
Meissner, Barbara; Warner, Adam; Wong, Kim et al. (2009) An integrated strategy to study muscle development and myofilament structure in Caenorhabditis elegans. PLoS Genet 5:e1000537
Watson, Joseph D; Wang, Shenglong; Von Stetina, Stephen E et al. (2008) Complementary RNA amplification methods enhance microarray identification of transcripts expressed in the C. elegans nervous system. BMC Genomics 9:84
Fox, Rebecca M; Watson, Joseph D; Von Stetina, Stephen E et al. (2007) The embryonic muscle transcriptome of Caenorhabditis elegans. Genome Biol 8:R188
Von Stetina, Stephen E; Watson, Joseph D; Fox, Rebecca M et al. (2007) Cell-specific microarray profiling experiments reveal a comprehensive picture of gene expression in the C. elegans nervous system. Genome Biol 8:R135
Von Stetina, Stephen E; Fox, Rebecca M; Watkins, Kathie L et al. (2007) UNC-4 represses CEH-12/HB9 to specify synaptic inputs to VA motor neurons in C. elegans. Genes Dev 21:332-46
Denton, Jerod; Nehrke, Keith; Yin, Xiaoyan et al. (2006) Altered gating and regulation of a carboxy-terminal ClC channel mutant expressed in the Caenorhabditis elegans oocyte. Am J Physiol Cell Physiol 290:C1109-18
Touroutine, Denis; Fox, Rebecca M; Von Stetina, Stephen E et al. (2005) acr-16 encodes an essential subunit of the levamisole-resistant nicotinic receptor at the Caenorhabditis elegans neuromuscular junction. J Biol Chem 280:27013-21

Showing the most recent 10 out of 23 publications