The EM core of this Program Project has two related purposes - to support instrumentation essential for theresearch proposed in the four projects of this grant and to facilitate application of the methods being developedin these projects to collaborative and independent efforts. The principal components of the core are the electronmicroscopy facilities at Harvard Medical School (HMS) and at Brandeis University. The HMS facility willhouse four FBI microscopes: a Tecnai F20, a Tecnai T12, a CM10, and a Polara F30. The F30, the F20, and theT12 microscopes are equipped with cryo-stages; the F30 and the F20 have field-emission guns. The Brandeisfacility also houses four FBI instruments: a Morgagni M268, and EM20, a CM12, and a Tecnai F30. The lastthree have cryo-stages; the F30 has a field-emission gun and an energy filter. The core supports the operation ofthese microscopes and the salary of a facilities manager at each location, as well as necessary ancillaryequipment. The core also has a computational component. It maintainsand upgrades all standard EM software,including program suites such as FREALIGN, IMAGIC, and SPIDER, through a structural biologycomputational grid (SBGrid) established by the Center for Molecular and Cellular Dynamics at HMS. Finally,by providing EM and computational facilities, the core allows the P.I.'s of these projects to collaborate withother groups at various institutionsand to train postdoctoral fellows and students in the relevant methods.Relevance: Electron microscopy is the key experimental tool that bridges between low- and high-resolutionstructural methods. Large assemblies will be targets of future interventions in human disease, and electronmicroscopy will be central for relating structure and function of these complexes
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