The objective of this project is to determine the role of specific antibodies and non-specific cell receptor analogs in breast milk in protecting infants from rotavirus infection.
The specific aims are as follows: 1) Determine the serotype distribution of asymptomatic and symptomatic rotavirus infection in breastfed and formula fed infants; 2) Evaluate the association between milk antibodies and rotavirus serotypes in infants who acquire asymptomatic and symptomatic infection with rotavirus; 3) Characterize intestinal secretory IgA (sIgA) antibody response to rotavirus proteins of the infecting serotype during the course of natural rotavirus infection and evaluate cross-reaction with other rotavirus serotypes including those of the major rotavirus vaccine strains. Associations will be examined between intestinal immunity and type of feeding during naturally acquired asymptomatic and symptomatic rotavirus infection; 4) Determine if immunization protects against rotavirus infection: a) Demonstrate in mice the ability of maternal milk antibodies (natural and immunization induced) to different rotavirus antigens to protect suckling infant mice from homologous and heterologous rotavirus infection. b) Identify a population of pregnant women and, following postpartum immunization of these women with rotavirus vaccine, determine the systemic and milk antibody response and the protection of milk and intestinal secretory antibodies against naturally acquired infection in breastfed infants; 5) Characterize non-immune factors in milk which may interfere with rotavirus infection. The importance of this subproject is that it will permit us to define the roles of milk antibodies against specific rotavirus proteins and cell receptor analogs in protection of infants from rotavirus infection.

Project Start
Project End
Budget Start
Budget End
Support Year
12
Fiscal Year
1991
Total Cost
Indirect Cost
City
Houston
State
TX
Country
United States
Zip Code
77225
Reed, Benjamin D; Schibler, Kurt R; Deshmukh, Hitesh et al. (2018) The Impact of Maternal Antibiotics on Neonatal Disease. J Pediatr 197:97-103.e3
Young, Bridget E; Patinkin, Zachary W; Pyle, Laura et al. (2017) Markers of Oxidative Stress in Human Milk do not Differ by Maternal BMI But are Related to Infant Growth Trajectories. Matern Child Health J 21:1367-1376
Dingess, Kelly A; Valentine, Christina J; Ollberding, Nicholas J et al. (2017) Branched-chain fatty acid composition of human milk and the impact of maternal diet: the Global Exploration of Human Milk (GEHM) Study. Am J Clin Nutr 105:177-184
He, YingYing; Lawlor, Nathan T; Newburg, David S (2016) Human Milk Components Modulate Toll-Like Receptor-Mediated Inflammation. Adv Nutr 7:102-11
Vanchiere, John A; Carillo, Berenice; Morrow, Ardythe L et al. (2016) Fecal Polyomavirus Excretion in Infancy. J Pediatric Infect Dis Soc 5:210-3
Ward, Doyle V; Scholz, Matthias; Zolfo, Moreno et al. (2016) Metagenomic Sequencing with Strain-Level Resolution Implicates Uropathogenic E. coli in Necrotizing Enterocolitis and Mortality in Preterm Infants. Cell Rep 14:2912-24
Newburg, David S; Ko, Jae Sung; Leone, Serena et al. (2016) Human Milk Oligosaccharides and Synthetic Galactosyloligosaccharides Contain 3'-, 4-, and 6'-Galactosyllactose and Attenuate Inflammation in Human T84, NCM-460, and H4 Cells and Intestinal Tissue Ex Vivo. J Nutr 146:358-67
He, YingYing; Liu, ShuBai; Kling, David E et al. (2016) The human milk oligosaccharide 2'-fucosyllactose modulates CD14 expression in human enterocytes, thereby attenuating LPS-induced inflammation. Gut 65:33-46
Payne, Daniel C; Currier, Rebecca L; Staat, Mary A et al. (2015) Epidemiologic Association Between FUT2 Secretor Status and Severe Rotavirus Gastroenteritis in Children in the United States. JAMA Pediatr 169:1040-5
Taft, Diana H; Ambalavanan, Namasivayam; Schibler, Kurt R et al. (2015) Center Variation in Intestinal Microbiota Prior to Late-Onset Sepsis in Preterm Infants. PLoS One 10:e0130604

Showing the most recent 10 out of 292 publications