Abstract

This application proposes a comprehensive effort to generate overlapping yeast artificial chromosome (YAC) clones stretching from the class II MHC locus HLA-DRb to the class I MHC genes as well as approximately 500-700 kb of previously unmapped DNA. A detailed restriction may of the cluster will be prepared and the newly cloned sequences will be analyzed for the presence of transcribed regions indicative of functional genes. In addition, probes derived at regular intervals along the cluster will be used to compare the structures of the MHC of individuals bearing disease-associated MHC extended haplotypes and normal individuals. The studies will permit a more complete analysis of the evolution of the MHC and may permit a better understanding of the function of the class III concerning the nature of extended MHC haplotypes of MHC-associated susceptibility to development of autominnume disease.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Research Program Projects (P01)
Project #
8P01HG000306-03
Application #
3878844
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Type
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130