Abstract

The Program Project core for """"""""Membrane Bound Enzymes in heart and Other Tissues"""""""" provides central support for all individual projects of this grant. Large scale preparations of mitochondria from beef heart as well as preparation of bacterial membranes and electron transport particles. Core personnel aid in purification of proteins used by individual projects in their research programs. In addition, oligonucleotides used for site- directed mutagenesis and other genetic manipulations are generated by the core. Administrative support for all individual projects is also the responsibility of the core personnel.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL016251-25
Application #
6109401
Study Section
Project Start
1999-07-01
Project End
2002-06-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
25
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Cecchini, Gary; Schroder, Imke; Gunsalus, Robert P et al. (2002) Succinate dehydrogenase and fumarate reductase from Escherichia coli. Biochim Biophys Acta 1553:140-57
Tornroth, Susanna; Yankovskaya, Victoria; Cecchini, Gary et al. (2002) Purification, crystallisation and preliminary crystallographic studies of succinate:ubiquinone oxidoreductase from Escherichia coli. Biochim Biophys Acta 1553:171-6
Ackrell, Brian A C (2002) Cytopathies involving mitochondrial complex II. Mol Aspects Med 23:369-84
Efimov, I; Cronin, C N; McIntire, W S (2001) Effects of noncovalent and covalent FAD binding on the redox and catalytic properties of p-cresol methylhydroxylase. Biochemistry 40:2155-66
Leger, C; Heffron, K; Pershad, H R et al. (2001) Enzyme electrokinetics: energetics of succinate oxidation by fumarate reductase and succinate dehydrogenase. Biochemistry 40:11234-45
Sablin, S O; Ramsay, R R (2001) Substrates but not inhibitors alter the redox potentials of monoamine oxidases. Antioxid Redox Signal 3:723-9
Maklashina, E; Rothery, R A; Weiner, J H et al. (2001) Retention of heme in axial ligand mutants of succinate-ubiquinone xxidoreductase (complex II) from Escherichia coli. J Biol Chem 276:18968-76
Chiu, H J; Johnson, E; Schroder, I et al. (2001) Crystal structures of a novel ferric reductase from the hyperthermophilic archaeon Archaeoglobus fulgidus and its complex with NADP+. Structure 9:311-9
Iverson, T M; Luna-Chavez, C; Schroder, I et al. (2000) Analyzing your complexes: structure of the quinol-fumarate reductase respiratory complex. Curr Opin Struct Biol 10:448-55
Luna-Chavez, C; Iverson, T M; Rees, D C et al. (2000) Overexpression, purification, and crystallization of the membrane-bound fumarate reductase from Escherichia coli. Protein Expr Purif 19:188-96

Showing the most recent 10 out of 164 publications