Abstract

In the """"""""07"""""""" year, (a) From X-ray scattering and EM image reconstructions, further attempts will be made to infer the attitude in which myosin subfragment 1 particles attach to regulated actin filaments. (b) Attempts will be made to record and interpret fluctuations arising from stochastic absorption/desorption of epsilon-ATP to S-1 active sites, using a quencher in the bulk phase. (c) Attempts will be made to measure distances from myosin S-1 """"""""landmarks"""""""" to the tryptophanes and the nucleotide of actin.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL016683-11
Application #
3097566
Study Section
Heart, Lung, and Blood Research Review Committee A (HLBA)
Project Start
1979-05-01
Project End
1989-04-30
Budget Start
1985-05-01
Budget End
1986-04-30
Support Year
11
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Muhlrad, A (1991) The isolated 21 kDa N-terminal fragment of myosin binds to actin in an ATP and ionic strength-dependent manner. Biochim Biophys Acta 1077:308-15
Curmi, P M; Stone, D B; Schneider, D K et al. (1991) Mechanism of force generation studied by neutron scattering. Adv Biophys 27:131-42
Mendelson, R A; Bivin, D; Curmi, P M et al. (1991) Recent neutron scattering studies of muscle contraction and its control. Adv Biophys 27:143-53
Muhlrad, A; Chaussepied, N (1990) Effect of nucleotides, actin and temperature on thermolysin digestion of myosin subfragment-1. Eur J Biochem 192:663-8
Kasprzak, A A; Chaussepied, P; Morales, M F (1989) Location of a contact site between actin and myosin in the three-dimensional structure of the acto-S1 complex. Biochemistry 28:9230-8
Chaussepied, P (1989) Interaction between stretch of residues 633-642 (actin binding site) and nucleotide binding site on skeletal myosin subfragment 1 heavy chain. Biochemistry 28:9123-8
Miyanishi, T; Borejdo, J (1989) Differential behavior of two cysteine residues on the myosin head in muscle fibers. Biochemistry 28:1287-94
Chaussepied, P; Kasprzak, A A (1989) Change in the actin-myosin subfragment 1 interaction during actin polymerization. J Biol Chem 264:20752-9
Borejdo, J (1989) Orientation of DNA in agarose gels. Biophys J 55:1183-90
Borejdo, J; Ortega, H (1989) Electrophoresis and orientation of F-actin in agarose gels. Biophys J 56:285-93

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