Abstract

Image acquisition, image processing, image analysis, and detection and interpretation of fluorescence signals are fundamental tools used in all of the projects. The availability of hardware and software to accomplish these tasks, and the availability of technical support to maintain and improve existing capabilities and to develop new processing methodologies is essential for the effective and timely completion of the proposed studies. The core includes support for confocal microscopy, flow cytometry, and support for the purchase of hardware to be used for video image processing and the development of software to facilitate extraction of information from experimental images. In addition, in the current application we propose to expand core capabilities to include Total Internal Reflectance Fluorescence Microscopy (TIRFM). Projects 2, 3 and 5 are developing new research directions that will benefit from this resource, and the remaining two projects, to a lesser extent, will benefit from the availability of these capabilities.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL018208-29A1
Application #
6932956
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
2004-07-01
Project End
2009-06-30
Budget Start
2004-07-01
Budget End
2005-06-30
Support Year
29
Fiscal Year
2004
Total Cost
$69,195
Indirect Cost

  Institution

Name
University of Rochester
Department
Type
DUNS #
041294109
City
Rochester
State
NY
Country
United States
Zip Code
14627

  Publications

Vats, Kanika; Marsh, Graham; Harding, Kristen et al. (2017) Nanoscale physicochemical properties of chain- and step-growth polymerized PEG hydrogels affect cell-material interactions. J Biomed Mater Res A 105:1112-1122
Henry, Steven J; Crocker, John C; Hammer, Daniel A (2016) Motile Human Neutrophils Sense Ligand Density Over Their Entire Contact Area. Ann Biomed Eng 44:886-94
Marsh, Graham; Waugh, Richard E (2016) A simple approach for bioactive surface calibration using evanescent waves. J Microsc 262:245-51
Rocheleau, Anne D; Wang, Weiwei; King, Michael R (2016) Effect of Pseudopod Extensions on Neutrophil Hemodynamic Transport Near a Wall. Cell Mol Bioeng 9:85-95
Svetina, Saša; Kokot, Gašper; Kebe, Tjaša Švelc et al. (2016) A novel strain energy relationship for red blood cell membrane skeleton based on spectrin stiffness and its application to micropipette deformation. Biomech Model Mechanobiol 15:745-58
Rocheleau, Anne D; Cao, Thong M; Takitani, Tait et al. (2016) Comparison of human and mouse E-selectin binding to Sialyl-Lewis(x). BMC Struct Biol 16:10
MacKay, Joanna L; Hammer, Daniel A (2016) Stiff substrates enhance monocytic cell capture through E-selectin but not P-selectin. Integr Biol (Camb) 8:62-72
Hind, Laurel E; Lurier, Emily B; Dembo, Micah et al. (2016) Effect of M1-M2 Polarization on the Motility and Traction Stresses of Primary Human Macrophages. Cell Mol Bioeng 9:455-465
Lim, Kihong; Hyun, Young-Min; Lambert-Emo, Kris et al. (2015) Visualization of integrin Mac-1 in vivo. J Immunol Methods 426:120-7
Beste, Michael T; Lomakina, Elena B; Hammer, Daniel A et al. (2015) Immobilized IL-8 Triggers Phagocytosis and Dynamic Changes in Membrane Microtopology in Human Neutrophils. Ann Biomed Eng 43:2207-19

Showing the most recent 10 out of 249 publications