The function of this core unit is to centralize the facilities and expertise of the major physical chemical and structural techniques utilized in the four project areas forming the Program Project. Facilities Provided by the Core Unit: 1) X-Ray Crystallography 2) Nuclear Magnetic Resonance Spectroscopy Nuclei: 13C, 1H, 2H, 31P, 15N and all other NMR nuclei 3) Calorimetry High sensitivity adiabatic differential scanning, titration 4) Electron Microscopy Negative staining, sectioning and morphological analysis 5) Structural Electron Microscopy and Image Processing 6) Protein Crystallization 7) Circular Dichroism Spectroscopy Near and far UV 8) Molecular Modeling, Graphics, and Molecular Dynamics 9) Crystallographic Computing 10) Surface Monolayer Techniques

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Heart, Lung, and Blood Initial Review Group (HLBP)
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Boston University
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Melchior, John T; Walker, Ryan G; Cooke, Allison L et al. (2017) A consensus model of human apolipoprotein A-I in its monomeric and lipid-free state. Nat Struct Mol Biol 24:1093-1099
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Mei, Xiaohu; Atkinson, David (2015) Lipid-free Apolipoprotein A-I Structure: Insights into HDL Formation and Atherosclerosis Development. Arch Med Res 46:351-60
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Gursky, Olga (2013) Crystal structure of ?(185-243)ApoA-I suggests a mechanistic framework for the protein adaptation to the changing lipid load in good cholesterol: from flatland to sphereland via double belt, belt buckle, double hairpin and trefoil/tetrafoil. J Mol Biol 425:1-16
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