Abstract

The projects listed below are combined by their focus on erythrocyte transglutaminase, a post-translationally acting protein modifying enzyme, capable of the rapid cross-linking of some of the membrane skeletal monomeric proteins. The topics are subdivided into the following categories with the specific aim of understanding the expression and functioning of this red cell enzyme in its broadest context. I.Protein modifications in the membranes of pathological red cells. II.A novel, in vitro, model for the precipitation of Hb onto the membrane (Heinz body formation). III.Exploring transglutaminase-reactive contact domains of isolated human red cell membrane proteins. IV.Interfering with membrane protein cross-linking and proteolysis in the human red cell by novel inhibitors. V.Thrombosis without thrombin in hemolytic diseases. VI.Molecular biology of red cell transglutaminase.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL045168-04
Application #
3758876
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
Northwestern University at Chicago
Department
Type
DUNS #
City
Evanston
State
IL
Country
United States
Zip Code
60201

  Publications

Koenig, Joyce M; Ballantyne, Christie M; Kumar, Ajith G et al. (2002) Vascular cell adhesion molecule-1 expression and hematopoietic supportive capacity of immortalized murine stromal cell lines derived from fetal liver and adult bone marrow. In Vitro Cell Dev Biol Anim 38:538-43
Swihart, A H; Mikrut, J M; Ketterson, J B et al. (2001) Atomic force microscopy of the erythrocyte membrane skeleton. J Microsc 204:212-25
Dutt, P; Hanenberg, H; Vik, T et al. (1997) A recombinant human fibronectin fragment facilitates retroviral mediated gene transfer into human hematopoietic progenitor cells. Biochem Mol Biol Int 42:909-17
Song, W; Wagle, N M; Banh, T et al. (1997) Wortmannin, a phosphatidylinositol 3-kinase inhibitor, blocks the assembly of peptide-MHC class II complexes. Int Immunol 9:1709-22
Pantazatos, D P; MacDonald, R I (1997) Site-directed mutagenesis of either the highly conserved Trp-22 or the moderately conserved Trp-95 to a large, hydrophobic residue reduces the thermodynamic stability of a spectrin repeating unit. J Biol Chem 272:21052-9
Mc Kiernan, A E; MacDonald, R I; MacDonald, R C et al. (1997) Cytoskeletal protein binding kinetics at planar phospholipid membranes. Biophys J 73:1987-98
Freie, B W; Dutt, P; Clapp, D W (1996) Correction of Fanconi anemia type C phenotypic abnormalities using a clinically suitable retroviral vector infection protocol. Cell Transplant 5:385-93
Moritz, T; Dutt, P; Xiao, X et al. (1996) Fibronectin improves transduction of reconstituting hematopoietic stem cells by retroviral vectors: evidence of direct viral binding to chymotryptic carboxy-terminal fragments. Blood 88:855-62
Orazi, A; Du, X; Yang, Z et al. (1996) Interleukin-11 prevents apoptosis and accelerates recovery of small intestinal mucosa in mice treated with combined chemotherapy and radiation. Lab Invest 75:33-42
Schafer, P H; Green, J M; Malapati, S et al. (1996) HLA-DM is present in one-fifth the amount of HLA-DR in the class II peptide-loading compartment where it associates with leupeptin-induced peptide (LIP)-HLA-DR complexes. J Immunol 157:5487-95

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