Abstract

The Cellular and Mouse Surgical Core (Core A) will provide 1. Dissociated adult and neonatal cardiomyocytes, 2. Cultured cells, 3. Micro-dissected canine heart tissues, and 4. Implantation and monitoring of ECG telemeters. Four of the Projects in the Program will required these services. Dissociation of cardiomyocytes is technically demanding (especially in mice), often resulting in unreliable access to cells. The Anderson laboratory has developed improved methods to reliably produce, and culture cardiomyocytes for use in these Projects. The core facility will streamline necessary tasks and centralize purchasing of reagents and location of incubators and sterile facilities to provide these Projects with reliable access to cells, and tissues. ECG telemetry provides information on arrhythmias in unrestrained and unanesthetized mice and is a significant improvement over previous approaches for monitoring conventional surface ECGs, but is technically demanding. ECG telemetry for mice in Projects 0009 and 0010 will be brought together with dissociation of cardiomyocytes to streamline activities that require similar technical (micro-surgical) skills and are performed by the same individuals.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL046681-14
Application #
7103452
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2005-08-01
Budget End
2006-07-31
Support Year
14
Fiscal Year
2005
Total Cost
$175,005
Indirect Cost

  Institution

Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212

  Publications

Yang, Tao; Smith, Jarrod A; Leake, Brenda F et al. (2013) An allosteric mechanism for drug block of the human cardiac potassium channel KCNQ1. Mol Pharmacol 83:481-9
Hayashi, Kenshi; Shuai, Wen; Sakamoto, Yuichiro et al. (2010) Trafficking-competent KCNQ1 variably influences the function of HERG long QT alleles. Heart Rhythm 7:973-80
Yang, Tao; McBride, Brian F; Leake, Brenda F et al. (2010) Modulation of drug block of the cardiac potassium channel KCNA5 by the drug transporters OCTN1 and MDR1. Br J Pharmacol 161:1023-33
Stepanovic, Svetlana Z; Potet, Franck; Petersen, Christina I et al. (2009) The evolutionarily conserved residue A653 plays a key role in HERG channel closing. J Physiol 587:2555-66
Yang, Tao; Chung, Seo-Kyung; Zhang, Wei et al. (2009) Biophysical properties of 9 KCNQ1 mutations associated with long-QT syndrome. Circ Arrhythm Electrophysiol 2:417-26
Potet, Franck; Petersen, Christina I; Boutaud, Olivier et al. (2009) Genetic screening in C. elegans identifies rho-GTPase activating protein 6 as novel HERG regulator. J Mol Cell Cardiol 46:257-67
Yang, Tao; Kanki, Hideaki; Zhang, Wei et al. (2009) Probing the mechanisms underlying modulation of quinidine sensitivity to cardiac I(Ks) block by protein kinase A-mediated I(Ks) phosphorylation. Br J Pharmacol 157:952-61
Yang, P; Koopmann, T T; Pfeufer, A et al. (2008) Polymorphisms in the cardiac sodium channel promoter displaying variant in vitro expression activity. Eur J Hum Genet 16:350-7
Watanabe, Hiroshi; Koopmann, Tamara T; Le Scouarnec, Solena et al. (2008) Sodium channel beta1 subunit mutations associated with Brugada syndrome and cardiac conduction disease in humans. J Clin Invest 118:2260-8
Grueter, Chad E; Abiria, Sunday A; Wu, Yunji et al. (2008) Differential regulated interactions of calcium/calmodulin-dependent protein kinase II with isoforms of voltage-gated calcium channel beta subunits. Biochemistry 47:1760-7

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