Abstract

The purpose of the this core facility is to provide photo-cleavable 'caged' compounds for use in subprojects within the Program. Compounds with known properties including caged chelators, caged pH probes, caged diacylglycerols and caged inositol phosphates will be made available in highly purified form. Several novel caged peptides, caged diacylglycerols and caged pH probes will be synthesized and characterized by chemical analysis, photolysis, and biochemical assays (enzyme or binding assays). In addition, chemical strategies will be explored for making certain compounds reversibly membrane permeant. The core will provide expertise in chemical synthesis, solid phase peptide synthesis, chemical analysis by nuclear magnetic resonance spectroscopy, UV/Vis absorption spectroscopy, mass spectroscopy, photochemistry and optics. Technical expertise in the use of caged compounds will also be provided including chromatographic analysis of purity, analysis of extents of photoconversion on photolysis, and optimization of photolysis optics for each experimental arrangement.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL047053-05
Application #
6110115
Study Section
Project Start
1999-01-01
Project End
2000-06-09
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
5
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Druckenbrod, Noah R; Powers, Patricia A; Bartley, Christopher R et al. (2008) Targeting of endothelin receptor-B to the neural crest. Genesis 46:396-400
Brickson, S; Fitzsimons, D P; Pereira, L et al. (2007) In vivo left ventricular functional capacity is compromised in cMyBP-C null mice. Am J Physiol Heart Circ Physiol 292:H1747-54
Stelzer, Julian E; Patel, Jitandrakumar R; Moss, Richard L (2006) Acceleration of stretch activation in murine myocardium due to phosphorylation of myosin regulatory light chain. J Gen Physiol 128:261-72
Stelzer, Julian E; Patel, Jitandrakumar R; Moss, Richard L (2006) Protein kinase A-mediated acceleration of the stretch activation response in murine skinned myocardium is eliminated by ablation of cMyBP-C. Circ Res 99:884-90
Balijepalli, Ravi C; Foell, Jason D; Hall, Duane D et al. (2006) Localization of cardiac L-type Ca(2+) channels to a caveolar macromolecular signaling complex is required for beta(2)-adrenergic regulation. Proc Natl Acad Sci U S A 103:7500-5
Stelzer, Julian E; Dunning, Sandy B; Moss, Richard L (2006) Ablation of cardiac myosin-binding protein-C accelerates stretch activation in murine skinned myocardium. Circ Res 98:1212-8
Martinez-Fernandez, Sergio; Hernandez-Torres, Francisco; Franco, Diego et al. (2006) Pitx2c overexpression promotes cell proliferation and arrests differentiation in myoblasts. Dev Dyn 235:2930-9
Stelzer, Julian E; Moss, Richard L (2006) Contributions of stretch activation to length-dependent contraction in murine myocardium. J Gen Physiol 128:461-71
Vatta, Matteo; Ackerman, Michael J; Ye, Bin et al. (2006) Mutant caveolin-3 induces persistent late sodium current and is associated with long-QT syndrome. Circulation 114:2104-12
Stelzer, Julian E; Larsson, Lars; Fitzsimons, Daniel P et al. (2006) Activation dependence of stretch activation in mouse skinned myocardium: implications for ventricular function. J Gen Physiol 127:95-107

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