PROJECT II - ENDOTHELIAL FACTORS, THE KIDNEY AND HYPERTENSION PROJECT SUMMARY/ABSTRACT A central theme of this PPG has been the renal-body fluid feedback control system in which the kidneys play a dominant role in the long-term regulation of body fluid volumes and arterial pressure. A common defect that has been found in all forms of hypertension examined to date is a hypertensive shift in the pressure natriuresis relationship (1-3). The overall objective of Project 2 is to determine pathophysiological mechanisms whereby obesity alters endothelial factors, renal hemodynamcis, pressure natriuresis, and blood pressure regulation in a rat model of pregnancy-induced hypertension (PIH), produced by chronic reductions in uterine perfusion pressure or chronic sFlt-1 excess (3-6). While recent studies have demonstrated adverse effects of obesity on placental function, the effects of obesity and metabolic factors such as leptin on the pathways that link placental ischemia, endothelial dysfunction, and maternal blood pressure are unknown (7-9). Based on our preliminary data and the work of others, we propose to test the central hypothesis that obesity and metabolic factors such as leptin enhance the blood pressure responses to placental ischemia in pregnant rats by enhancing placental and adipose tissue production of sFlt-1. In addition, we propose that obesity and metabolic factors such as leptin exacerbate the blood pressure responses to placental ischemia or chronic sFLt-1 excess by exacerbating TNF? and AT1-AA induced endothelial cell production of ET-1. We also propose that high fat diet induced obesity reduces placental perfusion by attenuating cytotrophoblast proliferation/migration and spiral artery remodeling during pregnancy by suppressing the Notch-2/JAG1 pathway. To test this hypothesis, arterial pressure will be examined in conscious, chronically instrumented rat models of preeclampsia produced by long-term reductions in uterine perfusion pressure (RUPP model) or by chronic sFlt-1 excess. We will examine the effects of obesity utilizing a genetic model of obesity which has a Mc4r mutation (MC4R+/-). In addition to animal models, in vitro placental explant cultures will be used to examine the effect of obesity on hypoxia-induced sFlt-1 production, and endothelial cell culture models to examine the effects of obesity on TNF? and AT1-AA-induced ET-1 production by endothelial cells. Cultured cytotrophoblasts, micro-CT, and Doppler velocimetry will be utilized to assess how obesity affects cytotrophoblast proliferation/migration, spiral artery remodeling and uterine artery resistance index. Thus, a wide range of molecular, biochemical, pharmacological, physiological, and imaging techniques as well as in vitro and in vivo models derived from various Cores will be used to test our central hypothesis.
PROJECT II - ENDOTHELIAL FACTORS, THE KIDNEY AND HYPERTENSION NARRATIVE Preeclampsia (PE) is estimated to affect 5-7% of all pregnancies and is a leading cause of maternal death and major contributor to maternal and perinatal morbidity. Strikingly, the incidence of PE has increased by 40% over the last several decades as a result of a significant increase in risk factors such as obesity. Obesity is a major epidemic in developed countries and the percentage of women who are obese or overweight has increased almost 60% in the last 30 years. Despite the fact that obesity is the leading attributable risk for PE, the pathophysiological mechanisms whereby obesity and related metabolic factors increase the risk for developing PE are unclear. The full elucidation of these mechanisms will hopefully lead to a more complete understanding of the etiology of preeclampsia and successful therapeutic intervention through the targeted disruption of novel pathways.
|Ma, Yonggang; Mouton, Alan J; Lindsey, Merry L (2018) Cardiac macrophage biology in the steady-state heart, the aging heart, and following myocardial infarction. Transl Res 191:15-28|
|Mouton, Alan J; DeLeon-Pennell, Kristine Y; Rivera Gonzalez, Osvaldo J et al. (2018) Mapping macrophage polarization over the myocardial infarction time continuum. Basic Res Cardiol 113:26|
|Meschiari, Cesar A; Jung, Mira; Iyer, Rugmani Padmanabhan et al. (2018) Macrophage overexpression of matrix metalloproteinase-9 in aged mice improves diastolic physiology and cardiac wound healing after myocardial infarction. Am J Physiol Heart Circ Physiol 314:H224-H235|
|Hinds Jr, Terry D; Stec, David E (2018) Bilirubin, a Cardiometabolic Signaling Molecule. Hypertension 72:788-795|
|Adeosun, Samuel O; Moore, Kyle H; Lang, David M et al. (2018) A Novel Fluorescence-Based Assay for the Measurement of Biliverdin Reductase Activity. React Oxyg Species (Apex) 5:35-45|
|Hall, Michael E; Jordan, Jennifer H; Juncos, Luis A et al. (2018) BOLD magnetic resonance imaging in nephrology. Int J Nephrol Renovasc Dis 11:103-112|
|Bakrania, Bhavisha A; Spradley, Frank T; Satchell, Simon C et al. (2018) Heme oxygenase-1 is a potent inhibitor of placental ischemia-mediated endothelin-1 production in cultured human glomerular endothelial cells. Am J Physiol Regul Integr Comp Physiol 314:R427-R432|
|Chade, Alejandro R; Williams, Maxx L; Guise, Erika et al. (2018) Systemic biopolymer-delivered vascular endothelial growth factor promotes therapeutic angiogenesis in experimental renovascular disease. Kidney Int 93:842-854|
|Clemmer, John S; Hester, Robert L; Pruett, W Andrew (2018) Simulating a virtual population's sensitivity to salt and uninephrectomy. Interface Focus 8:20160134|
|Granger, Joey P; Spradley, Frank T; Bakrania, Bhavisha A (2018) The Endothelin System: A Critical Player in the Pathophysiology of Preeclampsia. Curr Hypertens Rep 20:32|
Showing the most recent 10 out of 767 publications