The purpose of the Vector Production Core (Core B) is to develop and provide gene transfer reagents to members of the proposed Program efficiently and in a cost-effective manner. The research-grade vector development/production laboratory has been well established and centralized core component of the UCSD Gene Therapy Program and has demonstrated the benefit of such a core facility to the current program project as well as UCSD and other investigators through many venues. The Core laboratory has great expertise in the development and efficient preparation of a variety of high quality research-grade gene transfer vectors, including: adenovirus, multiple serotypes of adeno-associated virus (AAV), lentivirus, retrovirus (ecotropic, amphotropic, and VSV-G pseudotyped), VSV-G virosomes and plasmids. Since its inception in 1995, the Core has accommodated more than 780 requests, many of which have been development/preparation of multiple viral vectors. Technological innovation has also been an important function of the Core laboratory and it has made significant innovations to viral vectors, including improvement in vector production and purification procedures, development of double-strand DNA genome AAV vectors (scAAVs), tetracycline-inducible vectors, tissue-specific and tet-regulated vectors for shRNA/microRNA, and multiple gene expression from a single vector. Core B will work closely with project members, continue technological innovations and quickly incorporate new designs into the service function to provide the most efficient forms of gene transfer vectors to the proposed Program Project.
Every project within the proposed program involves intensive use of viral vectors for gene transfer and will require the expertise of the Core B. Core B will provide to the program participants with the most efficient and innovated form of gene transfer reagents and related services in a centralized manner. The resources are essential to the timely accomplishment of the goal of this program project.
|Suarez, Jorge; Cividini, Federico; Scott, Brian T et al. (2018) Restoring mitochondrial calcium uniporter expression in diabetic mouse heart improves mitochondrial calcium handling and cardiac function. J Biol Chem 293:8182-8195|
|Schilling, Jan M; Head, Brian P; Patel, Hemal H (2018) Caveolins as Regulators of Stress Adaptation. Mol Pharmacol 93:277-285|
|Giamouridis, Dimosthenis; Gao, Mei Hua; Lai, N Chin et al. (2018) Effects of Urocortin 2 Versus Urocortin 3 Gene Transfer on Left Ventricular Function and Glucose Disposal. JACC Basic Transl Sci 3:249-264|
|Hastings, Randolph H; Montgrain, Philippe R; Quintana, Rick A et al. (2017) Lung carcinoma progression and survival versus amino- and carboxyl-parathyroid hormone-related protein expression. J Cancer Res Clin Oncol 143:1395-1407|
|Gao, Mei Hua; Lai, N Chin; Giamouridis, Dimosthenis et al. (2017) Cardiac-directed expression of a catalytically inactive adenylyl cyclase 6 protects the heart from sustained ?-adrenergic stimulation. PLoS One 12:e0181282|
|Penny, William F; Hammond, H Kirk (2017) Randomized Clinical Trials of Gene Transfer for Heart Failure with Reduced Ejection Fraction. Hum Gene Ther 28:378-384|
|Egawa, Junji; Schilling, Jan M; Cui, Weihua et al. (2017) Neuron-specific caveolin-1 overexpression improves motor function and preserves memory in mice subjected to brain trauma. FASEB J 31:3403-3411|
|Breen, Ellen C; Scadeng, Miriam; Lai, N Chin et al. (2017) Functional magnetic resonance imaging for in vivo quantification of pulmonary hypertension in the Sugen 5416/hypoxia mouse. Exp Physiol 102:347-353|
|Chen, Chao; Li, Ruixia; Ross, Robert S et al. (2016) Integrins and integrin-related proteins in cardiac fibrosis. J Mol Cell Cardiol 93:162-74|
|Gao, Mei Hua; Lai, N Chin; Giamouridis, Dimosthenis et al. (2016) Cardiac-Directed Expression of Adenylyl Cyclase Catalytic Domain Reverses Cardiac Dysfunction Caused by Sustained Beta-Adrenergic Receptor Stimulation. JACC Basic Transl Sci 1:617-629|
Showing the most recent 10 out of 107 publications