The Analytical Core (Core B) will be organized to provide high quality, reproducible, low cost, automated high-throughput robotic analytical services to the four projects as well as the Animal Core (Core C). The core will be managed and staffed with experts who have demonstrated an ability to develop, engineer, and implement the assays necessary to support the proposed activities of the Analytical Core. The Analytical core will be organized around 4 major services/specific aims.
Specific Aim 1 : To provide high quality, reproducible automated assays to support Projects 1-3 and the Animal Core.
Specific Aim 2 : To provide genotyping services for projects #1 and #2. We will test for the presence of SNPs in GRK4, angiotensin converting enzyme (ACE l/D), aldosterone synthase, angiotensinogen, and ATIR using our previously published method {Bengra, 2002, 12446468} with a data reducfion method (Moore, Pub Med ID 12108579). Additional SNPs will be added as needs arise.
Specific Aim 3 : To provide cultured human renal proximal tubule cells for projects #1and #3 using a novel patent pending 3D cell culture method. We will also use our method to isolate individual renal proximal tubular cells (RPTCs) from fresh specimens of human urine in order to provide greater genetic diversity for studying human physiology, to correlate in vitro cellular responses to in vivo responses in the same patients as studied in Project 2.
Specific Aim 4 : To provide cellular imaging services to support projects #1 and #3. In addition, in support of project #2, we will perform human kidney slice culture and subsequent confocal analysis of DIR and DSR localization.

Public Health Relevance

Core B will develop and use novel analytical techniques to support the needs of the PPG, and to conduct its own hypothesis driven research. Undoubtedly, Core B will also publish its novel analytical developments in the form of patents and original publications.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Heart, Lung, and Blood Initial Review Group (HLBP)
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University of Virginia
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Muntner, Paul; Whelton, Paul K; Woodward, Mark et al. (2018) A Comparison of the 2017 American College of Cardiology/American Heart Association Blood Pressure Guideline and the 2017 American Diabetes Association Diabetes and Hypertension Position Statement for U.S. Adults With Diabetes. Diabetes Care 41:2322-2329
Ye, Zhengmeng; Lu, Xi; Deng, Yi et al. (2018) In Utero Exposure to Fine Particulate Matter Causes Hypertension Due to Impaired Renal Dopamine D1 Receptor in Offspring. Cell Physiol Biochem 46:148-159
Yang, Yang; Chen, Caiyu; Fu, Chunjiang et al. (2018) Angiotensin II type 2 receptor inhibits expression and function of insulin receptor in rat renal proximal tubule cells. J Am Soc Hypertens 12:135-145
Li, Fengmin; Yang, Jian; Villar, Van Anthony M et al. (2018) Loss of renal SNX5 results in impaired IDE activity and insulin resistance in mice. Diabetologia 61:727-737
Wang, Xiaoyan; Villar, Van Anthony; Tiu, Andrew et al. (2018) Dopamine D2 receptor upregulates leptin and IL-6 in adipocytes. J Lipid Res 59:607-614
Luo, Hao; Chen, Caiyu; Guo, Li et al. (2018) Exposure to Maternal Diabetes Mellitus Causes Renal Dopamine D1 Receptor Dysfunction and Hypertension in Adult Rat Offspring. Hypertension 72:962-970
Wu, Gengze; Jose, Pedro A; Zeng, Chunyu (2018) Noncoding RNAs in the Regulatory Network of Hypertension. Hypertension 72:1047-1059
Asico, Laureano D; Cuevas, Santiago; Ma, Xiaobo et al. (2018) Nephron segment-specific gene expression using AAV vectors. Biochem Biophys Res Commun 497:19-24
Tiu, Andrew C; Bishop, Michael D; Asico, Laureano D et al. (2017) Primary Pediatric Hypertension: Current Understanding and Emerging Concepts. Curr Hypertens Rep 19:70
Diao, Zhenyu; Asico, Laureano D; Villar, Van Anthony M et al. (2017) Increased renal oxidative stress in salt-sensitive human GRK4?486V transgenic mice. Free Radic Biol Med 106:80-90

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