Abstract

Analytical Core B Abstract The Analytical Core (Core B) will be organized to provide high quality, reproducible, low cost, automated high- throughput robotic analytical services to the three Projects. The Core will be managed and staffed with experts who have demonstrated an ability to develop, engineer, and implement the assays necessary to support the proposed activities of the Analytical Core. The Analytical Core will be organized around 5 major services/specific aims.
Specific Aim 1 : To provide high quality, reproducible automated assays to support Projects 1-3.
Specific Aim 2 : To provide genotyping services for Projects 1 and 2. We will test for the presence of single nucleotide polymorphisms (SNPs) in the subjects recruited under Project 2.
Specific Aim 3 : To provide human renal proximal tubule (RPT) cells as well as renal distal tubule (RDT) and renal collecting duct (RCD) cells for Projects 1 and 3. We will also isolate, immortalize and culture RPT, RTD and RCD cells from fresh human urine specimens from Project 2. Mouse urine cells will be obtained from Project 3.
Specific Aim 4 : To provide cellular imaging services to support Projects 1 and 3. In addition, in support of Project 2, we will study cells from the urine of subjects treated as outlined in the protocols of Project 2. We will also study cells isolated from mouse urine obtained from Project 3.
Specific Aim 5 : To provide flow cytometry services to support Projects 1-3. 1. Robotic Analytical Platforms High Throughput Assays High Content Assays 5. Flow Cytometry Accuri Urine Cell Assays Analytical Core B 2. Molecular Biology: SNPs for salt sensitivity and hypertension DNA extraction and Biobanking 4. Confocal Microscopy: FRET pH Assays Cytoplasmic cAMP, cGMP sensors Intracellular Na+ fluorescence 3. Cell Culture: RPT, RDT, RCD cell lines from human kidney tissue and urine Microcarrier-3D culture

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL074940-15
Application #
9949769
Study Section
Special Emphasis Panel (ZHL1)
Program Officer
OH, Youngsuk
Project Start
Project End
Budget Start
2020-06-01
Budget End
2021-05-31
Support Year
15
Fiscal Year
2020
Total Cost
Indirect Cost

  Institution

Name
University of Virginia
Department
Type
DUNS #
065391526
City
Charlottesville
State
VA
Country
United States
Zip Code
22904

  Publications

Ye, Zhengmeng; Lu, Xi; Deng, Yi et al. (2018) In Utero Exposure to Fine Particulate Matter Causes Hypertension Due to Impaired Renal Dopamine D1 Receptor in Offspring. Cell Physiol Biochem 46:148-159
Yang, Yang; Chen, Caiyu; Fu, Chunjiang et al. (2018) Angiotensin II type 2 receptor inhibits expression and function of insulin receptor in rat renal proximal tubule cells. J Am Soc Hypertens 12:135-145
Li, Fengmin; Yang, Jian; Villar, Van Anthony M et al. (2018) Loss of renal SNX5 results in impaired IDE activity and insulin resistance in mice. Diabetologia 61:727-737
Wang, Xiaoyan; Villar, Van Anthony; Tiu, Andrew et al. (2018) Dopamine D2 receptor upregulates leptin and IL-6 in adipocytes. J Lipid Res 59:607-614
Luo, Hao; Chen, Caiyu; Guo, Li et al. (2018) Exposure to Maternal Diabetes Mellitus Causes Renal Dopamine D1 Receptor Dysfunction and Hypertension in Adult Rat Offspring. Hypertension 72:962-970
Wu, Gengze; Jose, Pedro A; Zeng, Chunyu (2018) Noncoding RNAs in the Regulatory Network of Hypertension. Hypertension 72:1047-1059
Asico, Laureano D; Cuevas, Santiago; Ma, Xiaobo et al. (2018) Nephron segment-specific gene expression using AAV vectors. Biochem Biophys Res Commun 497:19-24
Muntner, Paul; Whelton, Paul K; Woodward, Mark et al. (2018) A Comparison of the 2017 American College of Cardiology/American Heart Association Blood Pressure Guideline and the 2017 American Diabetes Association Diabetes and Hypertension Position Statement for U.S. Adults With Diabetes. Diabetes Care 41:2322-2329
Tiu, Andrew C; Bishop, Michael D; Asico, Laureano D et al. (2017) Primary Pediatric Hypertension: Current Understanding and Emerging Concepts. Curr Hypertens Rep 19:70
Diao, Zhenyu; Asico, Laureano D; Villar, Van Anthony M et al. (2017) Increased renal oxidative stress in salt-sensitive human GRK4?486V transgenic mice. Free Radic Biol Med 106:80-90

Showing the most recent 10 out of 123 publications