Core B: Laboratory Testing CoreThe Testing Laboratory Core B has the goal to serve every project in the Program. It will provide an array ofassays assessing fibrinolysis, coagulation, anticoagulant activities, and plasma lipid profiles. The Core willalso provide assays of inflammatory cytokines, and adipokines as well as with established kits for mouseproteins associated with thrombosis, atherosclerosis and obesity. The specific assays that are available foreach of the projects are indicated below. Core B will also coordinate the high through-put screening of smallmolecules by the Center for Chemical Genomics.Project 1: Core B will perform analysis of plasma PAI-1 levels for both active and inactive PAI-1 and ofplasminogen activator antigen and activity levels. In addition, plasma lipid profiles will be routinelydetermined and immunoassays of various inflammatory cytokines as well as adipokines will be measured ina mutiplex format using the Luminex Technology. High through-put screening of small molecule compoundlibraries for the development of novel PAI-1 inactivating agents will also be performed through the Center forChemical Genomics.Project 2: Core B will perform analysis of plasma PAI-1 levels for both active and inactive PAI-1 and ofplasminogen activator antigen and activity levels. Murine plasmas will also be analyzed for increased risk forthrombosis by determining the levels of plasma coagulation, and anticoagulant proteins in various strains ofmice at baseline, and after venous thrombosis with or without hypercholesterolemia. D-dimer levels will alsobe measured. Procoagulant microparticles will also be measured as well as immunoassays of variousinflammatory cytokines.Project 3: Core B will assist the investigators off Project 3 by assessing platelet function including bleedingtimes and platelet aggregation in mice. Fibrinolytic parameters will be determined including PAI-1 andplasminogen activator levels. Immunoassays of various inflammatory cytokines will also be measured in amutiplex format using the Luminex Technology.Project 4: Core B will assist the investigators of Project 4 by developing novel, assays forcoagulation/fibrinolysis and platelet function in Zebrafish. These assays will include immunoassays andfunctional assays such as clotting times as well as factor assays.The overall goals of the Testing Laboratory Core B are to enhance the success of each individual project byproviding rigorous, standardized assays and to ensure that the combined Program is stronger than each ofits individual parts.
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