The Cardiac Physiology and Surgical Core (Core C) will be a focal point of the program by providing the infrastructure to support numerous experimental approaches to assess cardiac physiology and pathology, in the intact animal. Utilizing the vast experience and expertise of the Co-Director's, and well developed and proven protocols for the assessment of cardioprotection in multiple animal models, the Core will not only provide a consistent and reproducible service in support of the projects but will further participate in experimental design, prioritization of resources, and the interpretation of data. In addition, Core C will identify additional areas for collaboration across all projects as the program matures and thus be central to the interactive environment and comprehensive analysis of the overall platform as data is generated. The scientific core will provide a wide-range of services pertinent to the overall success of the program including in both mouse and porcine models: delivery of novel cell-free exosome therapeutics to the myocardium, in vivo induction of ischemia/reperfusion (IR) injury, the assessment of cardiac structure and function by multiple methodologies, and histological and biochemical assessment of myocardial injury. These services will provide the backbone for the major focus of this Program Project Grant to determine the impact of exosome therapy on the molecular pathogenesis of cardiac IR-injury.

Public Health Relevance

The goal of the Cardiac Physiology and Surgical Core (Core C) is to support all projects by providing expertise to carry out numerous experimental approaches to examine cardiac physiology and pathology in the intact animal. These services will provide the backbone for the major focus of this Program Project Grant to determine the impact of exosome therapy on the pathogenesis of cardiac ischemia-reperfusion injury (heart attack). Understanding these very important processes we hope will foster new treatments for heart disease.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL134608-04
Application #
9980466
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Program Officer
Wong, Renee P
Project Start
Project End
Budget Start
2020-09-01
Budget End
2021-08-31
Support Year
4
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Temple University
Department
Type
DUNS #
057123192
City
Philadelphia
State
PA
Country
United States
Zip Code
19122
Sluijter, Joost Petrus Gerardus; Davidson, Sean Michael; Boulanger, Chantal M et al. (2018) Extracellular vesicles in diagnostics and therapy of the ischaemic heart: Position Paper from the Working Group on Cellular Biology of the Heart of the European Society of Cardiology. Cardiovasc Res 114:19-34
Garikipati, Venkata Naga Srikanth; Kishore, Raj (2018) Induced Pluripotent Stem Cells Derived Extracellular Vesicles: A Potential Therapy for Cardiac Repair. Circ Res 122:197-198
Cheng, Zhongjian; Shen, Xinggui; Jiang, Xiaohua et al. (2018) Hyperhomocysteinemia potentiates diabetes-impaired EDHF-induced vascular relaxation: Role of insufficient hydrogen sulfide. Redox Biol 16:215-225
Gupta, Rajesh; Mackie, Alexander R; Misener, Sol et al. (2018) Endothelial smoothened-dependent hedgehog signaling is not required for sonic hedgehog induced angiogenesis or ischemic tissue repair. Lab Invest 98:682-691
Garikipati, Venkata Naga Srikanth; Kishore, Raj (2017) Cardiac progenitor cells: old is not always gold. J Physiol 595:6221-6222
Yue, Yujia; Garikipati, Venkata Naga Srikanth; Verma, Suresh Kumar et al. (2017) Interleukin-10 Deficiency Impairs Reparative Properties of Bone Marrow-Derived Endothelial Progenitor Cell Exosomes. Tissue Eng Part A 23:1241-1250